α-蒎烯衍生物对山核桃干腐病菌作用机理

α-pinene Derivative on Action Mechanism of Botryosphaeria fusisporae

为了探讨α-蒎烯衍生物3-甲氧基-4-羟基苯基-α-蒎烯马来酰亚胺基酰腙对病原菌的作用机理,采用平板扩散法研究了α-蒎烯衍生物对山核桃干腐病原菌(Botryosphaeria fusisporae)的抑制作用。结果表明:该衍生物可以抑制山核桃干腐病原菌生长,最大抑制率为63.37%,EC50为0.059 4 g·L-1,可导致山核桃干腐病菌菌丝体生长缓慢、质量减轻、菌液中p H值减小程度逐渐变大、电导率明显升高、大分子物质的吸光值增大、可溶性蛋白增加、过氧化物酶(POD)和过氧化氢酶(CAT)活性降低。说明α-蒎烯衍生物主要改变病原菌细胞膜的通透性,具有开发利用的潜力。

To explore the α-pinene derivative of 3-methoxy- 4-hydroxyphenyl-α-pinene maleimido acylhydrazone mechanism of pathogenic bacteria,we used the plate diffusion method to study the α-pinene derivative inhibition of Botryosphaeria fusisporae pathogens. The derivative can inhibit the growth of B. fusisporae pathogens,the largest inhibition was 63. 37%,EC50 was 0.059 4 g / L,which led to B. fusisporae pathogens mycelial growth slow weight loss,pH value decrease degree of bacteria gradually increased,the electrical conductivity significantly increased,the absorbance macromolecules increases,the soluble protein increased,POD and CAT activity decreased. The α-pinene derivative mainly changes the permeability of cell membrane pathogens with the potential development and utilization.