快速检测巯基化合物的新方法

A Novel Method for Rapid Detection of Thiol Compounds

本文利用巯基化合物对异硫氰酸荧光素(FITC)的荧光猝灭作用,建立了一种快速检测巯基化合物的新方法。实验中发现,巯基化合物能够猝灭FITC的荧光,据此发展了检测巯基化合物的新方法。本方法的用时非常短,在优化了pH、反应时间等条件后能在5min之内完成对巯基化合物的检测。在最佳条件下对实际样品的测试取得了很好的效果,且具有良好的选择性。对谷胱甘肽(GSH)、高半胱氨酸(Hcy)、半胱氨酸(Cys)、二硫苏糖醇(DTT)的检测限分别达到3.08、2.66、1.28和1.92μmol/L。

In this work,a novel method for rapid detection of thiol compounds was established using fluorescein isothiocyanate（ FITC） as fluorescence probe. The method was based on the quenching of the fluorescence of FITC by thiol compounds. Thiol compounds could be detected in 5 minutes at the optimized conditions including p H and reaction time. The method was used to detect glutathione（ GSH） in real samples and showed good selectivity and recovery. The limits of detection（ LOD） were 3. 08 μmol / L for glutathione（ GSH）,2. 66 μmol / L for homocysteine（ Hcy）,1. 28 μmol/L for cysteine（ Cys）,and 1. 92 μmol/L for dithiothreitol（ DTT）.