不同处理方法对金花茶组培苗不定根发生的影响

Effects of Different Processing Methods on Adventitious Rooting of Camellia nitidissima Tissue Culture Plantlets

[目的]筛选金花茶组培苗的最适生根培养条件。[方法]以金花茶组培苗为材料,研究不同激素处理、不同生根培养基、不同形态MW培养基对金花茶组培苗生根的影响。[结果]就促生根激素而言,500 mg/L IBA溶液浸泡处理效果较好,其生根率达82.5%,显著优于NAA;就基础培养基而言,MW培养基的生根效果更好,其生根率是1/2MS培养基的1.5倍;固体培养和滤纸桥液体培养同样都能诱导不定根产生,且生根率相当,不定根都较发达,侧根多。[结论]将金花茶无菌苗基部浸泡于500 mg/L IBA溶液后,转接于MW固体培养基进行生根培养,效果最好。

[Objective] The aim was to screen out the optimal rooting conditions for Camellia nitidissima tissue culture plantlets. [Method]With C. nitidissima tissue culture plantlets as materials,effects of different hormone treatments,different rooting culture medium and different morphology MW medium on rooting of C. nitidissima tissue culture plantlets were studied. [Result] The results showed that for promoting rooting hormone,the effect of 500 mg / L IBA solution soaking treatment is better,its rooting rate is as high as 82. 5%,significantly better than that of NAA;in terms of basic medium,MW rooting medium is better,the rooting rateis nearly 1. 5 times than that of 1 /2MS culture medium; solid cultivation and filter paper bridge liquid cultivation can both induce adventitious roots,and their rooting rates are nearly the same,both of them can induce many adventitious roots and lateral roots. [Conclusion] The rooting effect of soaking in 500 mg / L IBA solution and inoculating on MW culture medium is the best.