摘要
为建立小反刍兽疫病毒(PPRV)快速荧光RT-PCR检测方法,应用DNAstar软件对来自NCBI上的PPRVN基因序列进行比对分析,在保守区设置引物和探针,经条件优化,建立PPRV荧光RT-PCR检测方法。结果显示,该方法灵敏度高、特异性好、稳定性强,阳性结果与商用试剂盒的检测结果一致。结果表明该方法可用于PPRV的实验室检测。
In order to establish a rapid fluorescence RT-PCR assay for detection of Peste des Petits Ruminants virus（PPRV）,PPRV N gene sequences from NCBI were analyzed by DNAstar software. A pair of primers and a probe at the conservative site were designed. After optimization of conditions,the PPRV fluorescence RT-PCR detection method was established. The results showed that the method was of high sensitivity,good specifi city and good stability. The results of positive samples were consistent with commercial kits,indicated that this method could be used for PPRV detection in laboratory.
出处
《中国动物检疫》
CAS
2016年第6期72-76,共5页
China Animal Health Inspection
