响应面法优化藜麦多酚提取工艺的研究

Optimization of extraction technology of quinoa polyphenols by response surface methodology

为优化藜麦多酚提取工艺,以内蒙古种植的藜麦为实验材料,多酚得率为考察指标,研究乙醇浓度、料液比、提取温度和提取时间四个因素对藜麦多酚得率的影响。在单因素实验基础上,通过Box-Behnken实验设计方案优化藜麦多酚的最佳提取条件。实验结果表明,藜麦多酚的最佳提取工艺条件为:乙醇浓度49%,料液比1∶26(g/m L),提取温度73℃,提取时间62 min。在此条件下,藜麦多酚得率为(226.77±1.94)mg/100 g,优化后的提取工艺对藜麦多酚的提取有一定的指导意义。

To optimize the conditions for the extraction of polyphenols,using quinoa which grown in Inner Mongolia as raw material,with yield as test index to extract polyphenols.The effects of ethanol concentration,material liquid ratio,extraction temperature and time on yield of quinoa polyphenols were investigated. Experimental factors and their levels were determined by single factor test. Subsequently,the Box- Behnken experimental design was performed to optimize the conditions for quinoa polyphenols extraction. The results indicated the optimum conditions for quinoa polyphenols extraction were obtained as follows： ethanol concentration was 49%,material liquid ratio was 1∶ 26 g / m L,extraction temperature was 73 ℃,extraction time was 62 min.Under these conditions,the best yield of quinoa polyphenols was（ 226.77 ± 1.94） mg /100 g. The optimized extraction technology applied guiding significance for the quinoa polyphenols extraction.