生姜提取物对苯并芘染毒大鼠抗氧化酶及Ⅱ相解毒酶活性的影响

Effects of ginger extract on activities of antioxidant enzymes and phase II detoxifying enzymes in benzo( α ) pyrene- treated rats

探讨生姜提取物对苯并芘染毒大鼠抗氧化酶及II相解毒酶的影响。用不同剂量的生姜提取物(100、200和400 mg/kg(bw)对Wistar大鼠连续灌胃14 d后,一次性腹腔注射20 mg/kg·bw的苯并芘染毒,检测肝脏和肾脏中的抗氧化酶和II相解毒酶的活性。结果表明,三个剂量的生姜提取物灌胃处理对大鼠体重无显著影响(p>0.05),同时对肝、肺和肾的脏体比也无显著影响(p>0.05)。与苯并芘处理组相比,200和400 mg/kg·bw的生姜提取物能明显提高肝脏中谷胱甘肽过氧化物酶(GPx)和过氧化氢酶(CAT)的活力,以及肝和肾中醌还原酶(NQO1)和谷胱甘肽硫转移酶(GST)的活力(p<0.05)。以上结果表明,生姜提取物能显著提高大鼠肝脏中抗氧化酶及II相解毒酶的活性。

The aim of this study was to investigate the effects of ginger extract on activities of antioxidant enzymes and phase II enzymes in benzo（ α） pyrene（ Bα P）- treated rats.Wistar rats were fed by gavage with different doses of ginger extract 100,200 and 400 mg / kg·bw for 14 days. After 14 day of feeding,20 mg / kg·bw of Bα P was intraperitoneally injected to the rats.Afterwards,the animals were sacrificed and their livers and kidneys were collected for analysis of antioxidant enzymes and phase II enzymes. Results showed that three doses of ginger extract did not significantly affect body weight of the rats（ p〉0.05）,as well as the organs to body weight ratios（ p〉0.05）. Compared with the BαP group,both 200 and 400 mg /kg·bw of ginger extract significantly elevated the activities of glutathione peroxidase（ GPx） and catalase（ CAT） in liver,as well as the activities of quinone reductase（ NQO1） and glutathione S-transferase（ GST） in liver and kidney（ p〈0.05）.These results suggested that ginger extract could improve the activities of antioxidant enzymes and phase II enzymes in rats.