两种合成活性肽对肉鸡免疫功能的影响研究

Effect of Two Synthetic Active Peptides on Immune Function of Broilers

本试验旨在研究两种合成活性肽对肉鸡免疫功能的影响。1440只1 d AA肉鸡随机分为8个处理,设置2个对照组(空白对照组及杆菌肽锌组),试验组饮水供给两种肽(均设置3个剂量:10、20、40μg/kg体重),每组设6个重复,试验期42 d。结果表明:40μg/kg体重肽I和肽Ⅱ可显著提高21 d肉鸡血清新城疫抗体效价(P<0.05),显著提高21、42 d鸡血清Ig G水平(P<0.05);3个剂量肽I可显著提高血清Ig M水平(P<0.05),40μg/kg体重肽Ⅱ对42 d鸡亦有此作用(P<0.05)。21 d时各试验组血清IL-2含量均显著高于两个对照组(P<0.05或P<0.01),42 d时40μg/kg体重肽I组和肽Ⅱ组血清IL-2及IFN-r含量显著提高(P<0.01或P<0.05)。40μg/kg体重肽I和肽Ⅱ可显著提高42 d鸡血清溶菌酶含量(P<0.05),40μg/kg体重肽I可显著21 d鸡血清补体C3含量(P<0.05)。20μg/kg体重肽Ⅱ和肽I分别显著提高了21 d鸡法氏囊指数和42 d鸡胸腺指数(P<0.05)。可见,2种合成活性肽均有提高肉鸡免疫功能活性,均以饮水供给40μg/kg体重效果最好。

Two synthetic active peptides were studied to investigate their effe ct on immune function of broilers in this experiment.1440 1-day-old AA broilers were randomly divided into eight groups including two control groups（blank control group and bacitracin zinc group） and trial groups.Experimental groups with 6 replicates in each group were given two peptides by drinking supply with 3 doses（10,20,40 μg/kg body weight）.The test lasted 42 d.The results showed that：The serum Newcastle disease antibody titers of 21 d broilers were increased significantly by 40 μg/kg body weight peptide I and peptide Ⅱ（P 〈0.05）,and the serum Ig G levels of 21 d and 42 d broilers were increased significantly（P〈0.05）.The serum Ig M level was increased significantly by peptide I with three doses（P〈0.05 or P〈0.01）,and 40 μg/kg body weight peptideⅡhad the same effect on 42 d broilers（P〈0.05）.The serum IL-2 contents of all test groups were significantly higher than that of the two control groups at 21d（P〈0.05 or P〈0.01）.The serum IL-2 and IFN-r contents were also increased significantly in groups of 40 μg/kg body weight peptide I and peptide Ⅱ（P〈0.05 or P 〈0.01）.The serum lysozyme content of 42 d broilers was significantly improved by 40 μg/kg of body weight peptide I and peptide Ⅱ（P 〈0.05）.The serum complement C3 content of 21 d broilers was significantly improved by40 μg/kg body weight peptide I（P 〈0.05）.The bursal index of 21 d broilers was increased significantly by 20 μg/kg body weight peptideⅡ（P〈0.05）,and the thymus index of 42 d broilers was also improved by 20 μg/kg of body weight peptide I（P 〈0.05）.So the conclusion was that the two synthetic peptides had the activity of improving the immune function of broilers,and the best dose of them was 40 μg/kg body weight.