白蜡虫雄虫真蛹转录组分析

Transcriptome Analysis of Male White Wax Scale Pupae

[目的]蛹期对于白蜡虫雄虫发育和交配具有重要意义,但目前缺乏对于白蜡虫真蛹基因转录情况的分析,本研究旨在获得白蜡虫真蛹转录组数据,了解其转录组特征。[方法]采用Illumina Hi Seq 2000进行高通量测序,并进行生物信息学分析;采用荧光定量PCR(qRT-PCR)检测3个热激蛋白基因(hsp)在不同虫态中的表达动态。[结果]共获得63 957条unigene、63 272个开放阅读框(ORF)、9 561个SSR分子标记,unigene平均长度674 bp,共有14 327条unigene获得了注释,Gene Ontology(GO)注释和Kyoto Encyclopedia of Genes and Genomes(KEGG)注释分析表明,很多基因参与的功能与白蜡虫蛹期的生理特征吻合。[结论]该转录组数据为白蜡虫基因功能鉴定和蛋白组学分析研究奠定了数据基础。

Objective]To obtain the transcriptomic data and characterize the transcriptome of pupal stage of male white wax scale insect,Ericerus pela.[Method]The high-throughput sequencing was performed by Illumina HiSeq 2 000,and bioinformatics analysis were carried out subsequently.Three heat shock protein genes （hsps）were se-lected for expression profile analysis cross different developmental stages using quantitative real-time PCR （qRT-PCR）.[Result]The transcriptomic sequencing generated 63 957 unigenes,63 272 open reading frame （ORF）, and 9 561simple sequence repeat （SSR）markers.The average length of the unigenes was 674 bp.A total of 14 327 unigenes were annotated in different databases.The Gene Ontology （GO）and Kyoto Encyclopedia of Genes and Genomes （KEGG）analysis showed that the putative function of many genes coincided the physiological charac-terization of pupal stage.[Conclusion]The data and analysis of the transcriptome could lay a foundation for further gene functional research and proteomic analysis of E.pela.