PAEs类化合物对雄性小鼠的联合致毒作用

Study on synergistic toxicity of phthalic acid esters chemicals in male mice

为了探讨邻苯二甲酸酯(phthalic acid esters,PAEs)类化合物的联合毒性作用,将6种PAEs类化合物配制成等质量比的混合物,以低、中、高剂量(40,400,4 000 mg·kg^(-1))对雄性小鼠灌胃染毒,观察雄性小鼠骨髓微核形成、精子畸形、血清及肝脏、睾丸等指征的变化,并与相同剂量处理的单一邻苯二甲酸二丁酯(DBP)组进行比较,以评价混合PAEs组的联合毒性作用。结果显示:高剂量处理时,混合PAEs组的雄性小鼠骨髓微核率及精子畸形率显著高于单一DBP组;中剂量处理时,混合PAEs组染毒雄性小鼠30 d后的肝损伤及睾丸组织损伤程度也较单一DBP组明显上升,表明混合PAEs组对雄性小鼠的联合致毒作用较单一DBP组显著,不同PAEs类化合物间可能存在一定的协同效应。

In order to explore the synergistic toxicity of phthalate acid esters（ PAEs）,the bone marrow micronucleus formation,sperm deformity,liver and testis damage were carefully observed and determined on male mice,which were exposed to mixed PAEs chemicals with different levels of dose（ 40,400,4 000 mg·kg^（-1）） and single dibutyl phthalate（ DBP） with the same doses（ 40,400,4 000 mg·kg^（-1））. It was shown that the bone marrow micronucleus formation rate and sperm deformity rate were obviously higher in the mixed group with high-dose（ 4 000 mg·kg^（-1））PAEs than those in the single DBP treatment. Besides,the damage of liver and testis was also higher in the mixed group with moderate-dose（ 400 mg·kg^（-1）） PAEs than that in the single DBP treatment after 30 d. As mentioned above,the mixed PAEs exhibited more severe toxic effects than single DBP treatment,which suggested that there might be certain synergistic effect among PAEs chemicals.