大鼠腮腺组织放射后差异表达基因谱的构建及生物信息学分析

The Construction of Differentially Expressed Genes Spectrum of Parotid Gland Tissue In Rats After Radiation and Bioinformatics Analysis

目的探讨全基因芯片对放射后腮腺组织表达谱的差异及相关生物信息学的重要基因、通路。方法选用SD大鼠12只,随机分为空白组和放射组,每组各6只,一次性给予放射组60Co–γ射线15Gy的局部照射,空白组大鼠只腹腔注射10%水合氯醛。照射后2 h,无菌条件下迅速取出大鼠腮腺,投入液氮冷却。将提取的腮腺组织的总RNA进行Cye Dye Labeling荧光标定及Phalanx One ArrayTM杂交反应,利用含有20717个基因的ROAv2 Rat Ooe Array全基因表达谱芯片进行差异基因的筛选,将|log2(Ratio)|≥1作为筛选阈值。对筛选出的差异基因进行GO功能分类和Pathway通路分析。结果差异表达基因913条,上调基因455个,下调基因458个。将差异基因的功能概括为13类,包括代谢、细胞死亡、转录、应激等相关基因。对913个基因的Pathway通路有显著改变的通路共有13条,包括75个差异表达基因,其中7个涉及13条改变通路:Akt1、Pik3r1、Pik3cg、Nfkbia、Kras、Mapk13、Casp8。结论^(60)Co–γ射线可使正常的大鼠腮腺组织中的基因表达水平发生改变。Akt1、Nfkbia、TPM1在放射后腮腺组织中的的基因表达改变有待于进一步研究。

Objective To investigate the gene chip, the difference of the expression of parotid gland tissue after radiation spectrum and related important genes and pathways of bioinformatics. Methods 12, only choose SD rats were randomly divided into blank group and radiation group, each group 6, 60 co radiation group-once gamma-ray gy 15 local irradiation, blank group rats by intraperitoneal injection of only 10% chloral hydrate. 2 h after irradiation, aseptic conditions quickly remove the parotid gland of rats, liquid nitrogen cooling. Parotid gland tissue extraction of total RNA Cye Dye fluorescent Labeling calibration and Phalanx One Array TM hybridization reaction, using contains 20717 genes ROAv2 Rat Ooe Array-#174;all gene expression profile chip for differences in gene screening, will ｜ log2（thewire） ｜ 1 as a screening or threshold. To GO to screen out the difference of gene function classification and Pathway Pathway analysis. Results 913 differentially expressed genes, raised 455 genes, cut 458 genes. The function of the genetic variations will be divided into 13 classes, including metabolism, cell death, transcription, stress related genes. Pathway Pathway of 913 genes significantly change access article 13, including 75 differentially expressed genes, including seven involves 13 change pathways： Akt1, Pik3r1,Pik3 cg, Nfkbia, Kras, Mapk13, Casp8. Conclusion 60 co-gamma rays can make the level of gene expression in the parotid gland tissue of rats with normal change. Akt1, Nfkbia and TPM1 gene expression changes of parotid gland tissue after radiation needs to be further research.