期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

节律基因Clock对胶质瘤C6细胞增殖的影响

Proliferation effect of circadian gene Clock on glioma cells
下载PDF
导出
摘要 目的探讨节律基因Clock对胶质瘤C6细胞增殖的影响及其机制。方法通过小片段干扰RNA(small interfering,siRNA)特异性沉默C6细胞Clock基因的表达。采用CCK-8试剂盒检测C6细胞的增殖情况,并利用流式细胞仪检测Clock基因沉默后C6细胞的细胞周期分布,通过荧光定量Q-PCR检测β-catenin和Tcf1mRNA的表达水平。结果与空白对照组和阴性对照组相比,SiClock转染组C6细胞增殖活力明显降低,S期细胞所占比例明显下降,同时β-catenin和Tcf1mRNA的表达也明显降低(均P<0.05)。结论干扰节律基因Clock表达,能够抑制胶质瘤细胞的生长。其机制可能是通过Wnt/β-catenin信号通路调节其作用。 Objective To investigate the effect of circadian gene Clock on glioma C6 cells and its mechanism.Methods The expression level of Clock gene in C6 cells were specifically knocked down by RNA interference.The effect of C6 cells on cell growth and proliferation was demonstrated with CCK-8assay.The cell distribution in cell cycle was detected by flow cytometry.The expression of β-catenin and Tcf1 were detected by Q-PCR.Results Compared with the blank control group and the negative control group,the cells transfected with SiClock had the lower rate of cellular proliferative capacity.Meanwhile,the proportion of S phase cells decreased obviously.The mRNA expression of β-catenin and Tcf1 were significantly reduced.Conclusion Clock gene knocked down inhibited the proliferation of glioma cell,which mechanism might involve the Wnt/β-catenin signaling pathway.
作者 杨振华 李小雪 王正荣 杨淑红 江舟 成姝婷 刘延友 汪宇辉 肖静
机构地区 四川大学华西基础医学与法医学院.卫生部时间生物学重点实验室
出处 《西部医学》 2016年第6期750-753,758,共5页 Medical Journal of West China
基金 国家自然科学基金(31371180) 国家自然科学青年基金(31500935)
关键词 节律基因 CLOCK C6 增殖 Circadian genes Clock C6 proliferation
分类号 R-33 [医药卫生] R739.41 [医药卫生—肿瘤]
  • 相关文献

参考文献23

  • 1Reppert S M, Weaver D R. Coordination of circadian timing in mammals [J]. Nature, 2002, 418(6901): 935-941.
  • 2Turek F W. Circadian rhythms [-J]. Recent progress in hormone research, 1994, 49:43-90.
  • 3Kwon P, Lunding J, Li W, et al. Night shift work and lung cancer risk among female textile workers in Shanghai, China [J]. Journal of occupational and environmental hygiene, 2015, 12(5): 334-341.
  • 4Madden M H, Anic G M, Thompson R C, et al. Circadian pathway genes in relation to glioma risk and outcome [J]. Canc- er causes & control : CCC, 2014, 25(1): 25-32.
  • 5Schernhammer E S, Razavi P, LI T Y, et al. Rotating night shifts and risk of skin cancer in the nurses' health study [J]. Journal of the National Cancer Institute, 2011, 103 (7): 662- 606.
  • 6Markt S C, Valdimarsdottir U A, Shui I M, et al. Circadian clock genes and risk of fatal prostate cancer [J]. Cancer causes & control : CCC, 2015, 26(1): 25-33.
  • 7Hu M L, Yeh K T, Lin P M, et al. Deregulated expression of circadian clock genes in gastric cancer [J]. BMC gastroenterolo- gy, 2014, 14:67.
  • 8Barnes J W, Tischkau S A, Barnes J A, et al. Requirement of mammalian Timeless for circadian rhythmicity [J]. Science, 2003, 302(5644): 439-42.
  • 9Panda S, Antoch M P, Miller B H, et al. Coordinated tran- scription of key pathways in the mouse by the circadian clock [J]. Cell, 2002, 109(3): 307-320.
  • 10Miller B H, Mcdearmon E L, Panda S, et al. Circadian and CLOCK-controlled regulation of the mouse transcriptome and cell proliferation [J]. Proc Natl Acad Sci U S A, 2007, 104(9): 3342-3347.

二级参考文献86

  • 1Reppert SM, Weaver DR. Coordination of circadian timing in mam- mals [ J ]. Nature, 2002,418 (6901) :935-941.
  • 2Morse D, Sassone-Corsi P. Time after time: inputs to and outputs from the mammalian circadian oscillators J ]. Trends Neurosei, 2002,25 ( 12 ) :632-637.
  • 3Reppert SM, Weaver DR. Molecular analysis of mammalian eircadi-an rhythms[J]. Annu Rev Physiol, 2001,63(4) :647-676.
  • 4Panda S, Antoeh MP, Miller BH, et al. Coordinated transcription of key pathways in the mouse by the circadian clock [ J ]. Cell, 2002,109(3) :307-320.
  • 5Lee CC. Tumor suppression by the mammalian period genes [ J ]. Cancer Causes Control, 2006,17 (4) :525-530.
  • 6Gekakis N, Staknis D, Nguyen HB, et al. Role of the clock pro- tein in the mammalian circadian mechanism[ J]. Science, 1998, 280(5369) :1564-1569.
  • 7Matsuo T, Yamaguehi S, Mitsui S, et al. Control mechanism of the circadian clock for timing of cell division in vivo[ J]. Science, 2003,302 ( 5643 ) : 255-259.
  • 8Miller BH, MeDearmon EL, Panda S, et al. Circadian and clock controlled regulation of the mouse transeriptome and cell prolifera- tion[ J ]. Proc Natl Acad Sci USA, 2007,104(9) :3342-3347.
  • 9Antoch MP, Gorbacheva VY, Vykhovanets O, et al. Disruption of the circadian clock due to the clock mutation has discrete effects on aging and carcinogenesis [ J ]. Cell Cyele, 2008,7 ( 9 ) : 1197- 1204.
  • 10Aaron EH, Yi CH, Zheng TZ, et al. Clock in breast tumorigene- sis: genetic, epigenetic, and transcriptional profiling analyses [ J ]. Cancer Res, 2010,70(4) :1459-1468.

共引文献7

西部医学
2016年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部