樟芝发酵产2-苯乙醇的条件优化

Optimization of Selected Growth Parameters Affecting 2-Phenylethanol Production in Submerged Cultures of Taiwanofungus camphoratus

以药用真菌樟芝(Taiwanofungus camphoratus)发酵液中的主要香气成分之一2-苯乙醇的含量为指标,利用单因素和正交试验对发酵条件进行优化,获得了樟芝发酵产2-苯乙醇的最优条件:培养基(g/L)为50葡萄糖,5酵母粉,1磷酸氢二钾,0.5硫酸镁,0.1 VB1,pH为5;装液量为120 mL/250 mL三角瓶;接种量20%;转速150r/min;26℃恒温培养7d;在此条件下樟芝发酵液中的2-苯乙醇含量达到1.24g/L。

Selected culture parameters affecting the production of 2-phenylethanol（2-PE）,an aroma compound with a rose-like fragrance that is widely used in the cosmetic,perfume,and food industries,in submerged cultures of Taiwanofungus camphoratus grown in 250 mL flasks have been investigated.Single factorial experiments and orthogonal testing revealed that 2-PE synthesis was maximal（1.24g/L）when the fungus was grown in a culture medium consisting of（g/L）50glucose,5yeast powder,1 K2HPO4,0.5MgSO4 and 0.1vitamin B1,and when the following conditions were applied：initial pH of the medium,pH5;volume of medium per 250 mL flask,120 mL;inoculum size,20%;flask rotation speed,150r/min;culture temperature,26 ℃;culture time,7d.