膜过滤法分离纯化刺芹侧耳提取液中海藻糖的工艺

Separation of Trehalose from Aqueous Extracts of Pleurotus eryngii Fruit Bodies Using Membrane Filtration

联合使用截留相对分子质量1000的超滤膜和270的纳滤膜从刺芹侧耳(Pleurotus eryngii)水提液中分离海藻糖。超滤膜操作压力为4kg/cm^2,同时纳滤膜操作压力为3kg/cm^2,经膜分离的相对分子质量270~1000部分溶液经浓缩结晶,海藻糖纯度可达93.3%。

Pleurotus eryngii fruit bodies（1kg）were extracted twice with ddH2O at 100℃for 2h（fruit body∶water ratio was 1∶15w/v for the first extraction and 1∶10w/v for the second）.Combined extracts（total volume-18L）were filtered through cheese cloth and the filtrate was separated into three fractions using a polyether sulphone membrane filter（PES1）（MWCO 1000Da）and a NF270nanofilter（MWCO 270 Da）.Pressures of 4kg/cm^2 and 3kg/cm^2 were applied when filtering through the PESI and NF270 membranes,respectively.The fraction passing through the PESI membrane but retained by the NF270 nanofilter was concentrated using a rotary evaporator and the trehalose concentration in this fraction was adjusted to 30%.Five volumes（v/v）of absolute ethanol were added and,after mixing,the solution was left to stand overnight at 4 ℃.Precipitated material was collected by suction filtration and re-crystallized from water∶absolute ethanol（1∶5,v/v）to give a trehalose sample of 93.3% purity.