蛋白质组学方法鉴定小鼠Barnes迷宫空间记忆相关蛋白

Identification of Spatial Memory Related Proteins by Proteomics in Mice

目的：通过Barnes Maze （ Barnes迷宫， BM）对小鼠进行空间记忆训练，随后用蛋白质组学方法鉴定BM空间记忆相关的海马蛋白并分析蛋白功能及参与的生物学过程。方法将小鼠分为实验组和对照组。应用BM对实验组小鼠进行空间记忆训练，蛋白质组学方法鉴定两组小鼠中表达水平不同的海马蛋白，分析空间记忆相关的蛋白及其功能。结果实验组小鼠学习训练后学习记忆能力明显强于对照组，到达目标孔的时间明显缩短[对照组：（116．0＆#177;5．5） s，实验组：（42．0＆#177;3．6） s； P＜0．05]。蛋白质组学分析结果表明9种海马蛋白的表达水平在记忆形成过程中显著下调，下调的蛋白按功能可分为4类：①细胞骨架；②能量代谢；③神经发育；④物质运输。结论这9种学习记忆相关蛋白可能通过调节细胞骨架、能量代谢、神经发育和物质运输等生物学过程进而影响了学习记忆能力。

Objective To identify the hippocampal proteins associated with Barnes maze （ BM） with proteomics and analyze their functions and the biological processes in which they are in-volved. Methods C57 BL/6 J mice were divided into experimental group and control group ran-domly. Trainings about spatial learning and memory were preformed in the BM in the experimental group. Hippocampal proteins with different expression levels were identified with proteomics in both groups and the functions of the spatial memory related proteins were analyzed. Results The time to arrive at the target hole was significantly shorter after BM training [control group （116. 0 ± 5. 5） s vs. experimental group （42. 0 ± 3. 6） s; P〈0. 05], suggesting that the learning and memory abil-ities were significantly increased in the experimental group as compared with the control group. Proteomics revealed that 9 hippocampal proteins were down-regulated significantly. These down-regulated proteins fell into 4 categories in terms of different functions： ①cytoskeleton-related protein; ② energy metabolism related protein; ③neurogenesis related protein; ④transport relat-ed protein. Conclusion The 9 proteins may affect learning and memory abilities via regulating cy-toskeleton, energy metabolism, neurogenesis and material transportation.