长链非编码RNA测序技术应用探讨

Application of High-Sequencing Techniques to Identification of LncR-NAs

长链非编码RNA （ long non-coding RNA， LncRNA）是一类转录本长度超过200 nt的RNA分子，由RNA聚合酶Ⅱ（ RNA polymeraseⅡ）加工合成，是基因组序列转录生成的产物。近年来，随着高通量技术的不断发展，越来越多的LncRNA逐渐被发现并成为研究热点。 LncRNA作为新兴分子标志物与肿瘤的发生、发展机制密切相关。目前运用于功能性LncRNA筛查的分子高通量新一代测序方法高效且节源，并广泛应用于基因检测。 RNA-seq、 RIP-seq及CLIP-Seq此3种高通量测序在LncRNA中的研究运用已经报道，文章旨在深入探讨技术特点以帮助发掘肿瘤分子标志物。

Long non-coding RNAs （ LncRNAs） are non-protein coding transcripts longer than 200 nucleotides. They are processed and synthesized by RNA polymerase Ⅱ. Numerous LncRNAs have been found recently with the development of high-throughput sequencing technique and they have become a hotspot of research. LncRNAs as emerging molecular markers are closely associated with the occurrence and development of tumors. The new generation sequencing method （ high-through sequencing） is efficient and energy-saving for screening LncRNAs. RNA-seq, RIP-seq and CLIP-Seq have been reported to sequence the LncRNAs. In this review, we discuss the features of these techniques in order to help identify the molecular markers of tumors.