四种果皮中橙皮苷含量的测定

Determination of Hesperidin Contents in Four Kinds of Peels

为建立一种测定橙皮、橘皮、柚皮及柠檬皮中橙皮苷含量的高效液相色谱法。以Symmertry Shield RP18柱为分离柱,甲醇–2.5%乙酸溶液(40:60 V:V)为流动相,柱温30℃,紫外检测器在波长283 nm条件下检测,一次进样7 min内完成分析过程。橙皮苷在2.0~100.0 mg/L范围内有良好的线性关系(R=0.9999);平均加标回收率为98.9%,检出限为2.0×10^(–3) g/kg;精密度实验(n=6)表明,橙皮苷峰面积的相对标准偏差(RSD)为0.05%。该方法简便快捷,可准确测定四种果皮中橙皮苷的含量。

In order to develop a method for determination of hesperidin contents in orange peel, citrus peel, pomelo peel and lemon peel by HPLC. Hesperidin was qualitatively analyzed through a Symmertry Shield RP18 column. The mobile phase was methanol–2.5% acetic acid（40：60 V：V） and the column temperature was 30 ℃. A UV detector detected at 283 nm and the separation was achieved within 7 min. The standard working curve of hesperidin had a good liner relation in the concentration ranging from 2.0 to 100.0 mg/L（R=0.9999）. The average recovery was 98.9% and detection limit was 2.0×10–3g/kg. The precision test（n=6） showed that the relative standard deviation（RSD） for the peak areas was 0.05%. This method was simple and rapid and could determine the content of hesperidin in four kinds of peels.