红雪茶多糖过氧化氢脱色工艺优化

Optimization for decolorization process of Lethariella spp.polysaccharides with hydrogen peroxide

【目的】优化红雪茶多糖过氧化氢(H_2O_2)脱色工艺条件,为红雪茶多糖的纯化及其资源深度开发提供技术支持。【方法】在单因素试验基础上,选择脱色时间、脱色温度、H_2O_2体积分数为影响因素,以多糖脱色率为考察指标,采用正交试验优化红雪茶多糖的H_2O_2脱色工艺,确定最优工艺参数。【结果】3个因素对红雪茶多糖脱色效果影响的排序为:H_2O_2体积分数>脱色温度>脱色时间,其中H_2O_2体积分数有极显著影响(P<0.01)。红雪茶多糖的最佳H_2O_2脱色工艺条件为:脱色温度55℃、脱色时间20 min、H_2O_2体积分数20%,在此条件下脱色率为81.55%。【结论】H_2O_2脱色法对红雪茶多糖的脱色效率较高,且成本低廉、工艺稳定,是红雪茶多糖脱色的可行方法。

【Objective】The present experiment was conducted to optimize decolorization process of Lethariella spp.polysaccharides with hydrogen peroxide（H_2O_2）, in order to provide theoretical and experimental basis for purification and further development of Lethariella spp. polysaccharides. 【Method 】Based on single-factor experiment, the conditions of decolorization process of Lethariella spp. polysaccharides were optimized by orthogonal test, with decolorization temperature and time, H_2O_2 volume fraction as influence factors, and decolorization rate as index, so as to determine optimum process parameters. 【Result 】The results showed that, the primary and secondary sequence of 3 factors affecting decolorization rate of Lethariella spp. polysaccharides were as follows： H_2O_2 volume fraction decolorization temperature decolorization time, especially H_2O_2 volume fraction with extremely significant influence（P0.01）. The optimal extraction conditions were as follows： decolorization temperature of 55 ℃, decolorization time of 20 min, and H_2O_2 volume fraction of20%. Under the optimal conditions, the decolorization rate could reach up to 81.55%. 【Conclusion】It is feasible that H_2O_2 is used to discolor Lethariella spp. polysaccharides, due to its low cost, stability and hight decolorization efficiency.