利用基因组发掘技术指导链霉菌SH-62中活性天然产物的分离及鉴定

Separation and Characterization of Bioactive Natural Products in Streptomyces sp. SH-62 by Genome Mining

通过生物信息学分析,利用基因组发掘技术发现链霉菌SH-62基因组中至少含有37个次级代谢产物的生物合成基因簇,除了有4个基因簇分别与已知的肠道菌素、潮霉素A、尼日利亚菌素和格尔德霉素的生物合成基因簇具有高度同源性以外,其他基因簇的功能鲜见报道。在不同发酵培养基上培养链霉菌SH-62,利用高分辨率的LC-MS对发酵产物进行分析,发现链霉菌SH-62确实能够产生肠道菌素、潮霉素A和尼日利亚菌素,从而证明了基因组发掘策略确实能够指导代谢产物的分离及鉴定。

There were at least 37 gene clusters for biosynthesis of secondary metabolites which were found in the genome of Streptomyces. sp. SH-62 by bioinformatics analyses. Besides 4 of them were highly homologous with the well-known biosynthetic gene clusters of enterocin, hygromycin A, nigericin and geldanamycin, respectively, biological functions of others had not been reported yet. Production of enterocin, hygromycin A and nigericin was detected by high-resolution LC-MS analyses of the Streptomyces. sp. SH-62 fermentation products in different media. It demonstrated that the genome mining strategy is feasible to direct detection and identification of metabolites.