大鼠脑出血后血肿周围组织中树突状细胞的免疫功能研究

Study of immune function of dendritic cells in perihematoma tissue of rats after intracerebral hemorrhage

目的观察实验性大鼠脑血肿周围组织树突状细胞(dendritic cells,DC)的分布情况,进一步分析脑出血后不同时相内,DC在局部中枢性神经组织中免疫功能的作用。方法制作实验性大鼠基底节区脑血肿模型,分别检测24h、48h、72h、5d和7d各时相点神经功能障碍、脑组织水肿情况及局部脑组织中IL-6和GM-CSF的浓度、阳性DC数量。结果出血组GM-CSF浓度在48h时增加,随后仍保持持续性上升。实验组IL-6浓度在术后48h达到高峰,随后下降;实验组DC在术后24h时观察到,并随时间的延长持续增加。结论实验大鼠脑出血周围组织中的微状态变化,将限制和影响DC在不同时相的作用。在脑出血前期促进脑组织损害,脑出血后期有利于局部脑组织修复。

Objective To observe the distribution of dendritic cells（DC）in perihematoma tissue through the establishment of intracerebral hemorrhage（ICH）model of rat,and to analyze the immune function of dendrite cells in the local central nervous tissue,in the different phases after ICH.Methods The model of ICH in the caudate nucleus of rat was produced;and the neurological dysfunction,cerebral edema,concentration of interleukin-6（IL-6）and granulocyte macrophage-colony stimulating factor（GM-CSF）in local brain tissue,count of DC-positive were detected at 24-hour,48-hour,72-hour,5-day and 7-day time points.Results The concentration of GM-CSF in ICH group began to ascend at 48-hour,and then remained persistent rose.The concentration of IL-6in the experimental group was reached the peak at 48-hour after surgery,and then decreased;DC was observed in the experimental group at 24-hour after operation,and continued to increasing with the time prolonged.Conclusion The changes of micro environmental conditions in perihematoma tissue of ICH model of rat will restrict and influence the effect of DC according different phases.It may aggravate the brain damage in the early stage of ICH,and promote the damage tissue repaired in late stage.