弥可保对糖尿病神经病理性疼痛大鼠背根神经节Nav1.7表达的影响被引量：4

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摘要目的探讨弥可保对链脲佐菌素（STZ）致糖尿病模型大鼠痛阈及背根神经节（DRG）中电压门控性钠离子通道亚型1.7（Nav1.7）表达的影响。方法健康雄性SD大鼠75只,体质量200-250g,随机分为空白对照组（C组）、模型组（DNP组）和弥可保组（DNP＋M组）,每组25只,其中模型组和弥可保组皮下注射STZ 75mg/kg制作糖尿病大鼠模型,剔除血糖低于16.7mmol/L的大鼠,14d后给予安慰剂和弥可保（600μg/kg）腹腔注射,1次/d,观察STZ注射前1d及注射后3、7、14、21、28d机械缩足反射阈值（mechanical withdrwal threshold,MWT）,行为学测试完成后,采集大鼠背根神经节（DRG）标本,用免疫组化和Western blot方法分别检测其钠离子通道Nav1.7蛋白表达水平。结果与对照组比较,模型组大鼠MWT降低,DRG中Nav1.7的表达上调,差异有统计学意义（P〈0.05）;与模型组比较,弥可保组大鼠MWT升高,DRG中Nav1.7的表达下调,差异有统计学意义（P〈0.05）。结论弥可保通过抑制糖尿病大鼠DRG中Nav1.7表达,从而缓解大鼠神经病理性疼痛。

作者张春玲任幼红

机构地区山东平度市人民医院内分泌科郑州大学第一附属医院康复科

出处《中国实用神经疾病杂志》 2016年第11期36-38,共3页 Chinese Journal of Practical Nervous Diseases

关键词弥可保糖尿病神经病理性疼痛背根神经节 Nav1.7

分类号 R-332 [医药卫生]

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