大豆球蛋白酶解物的抗氧化性及汞离子结合特性的研究

The antioxidant activities and Hg-binding properties of soy glycinin hydrolysates

以未还原大豆球蛋白水解物为对照,研究了还原大豆球蛋白胃酶酶解物的抗氧化性及汞离子结合特性。结果表明:还原蛋白酶解物巯基含量为117.5μmol/g,是未还原蛋白酶解物的76.8倍。还原蛋白酶解物分子质量更小,疏水性与未还原蛋白酶解物相差不大。还原蛋白酶解物对超氧阴离子自由基、羟基自由基、DPPH自由基清除的IC50分别为18、20和0.7 mg/m L,当质量浓度为2 mg/m L时,Fe3+还原能力为0.557,汞离子结合量为8.02 mg/g,还原蛋白酶解物的抗氧化性和汞离子结合能力显著优于未还原酶解物。说明还原大豆球蛋白酶解物具有一定的汞离子解毒潜能。

The antioxidant activities and Hg-binding properties of reduced soy glycinin hydrolysates were studies comparing with that of non-reduced soy glycinin hydrolysates. Results indicated that the content of sulfhydryl group was 120 μmol / g,which was 100-fold of that of non-reduced soy glycinin hydrolysates. The molecular weight of reduced hydrolysates was smaller than non-reduced hydrolysates and their hydrophobic distributions were similar. The IC50 of Super oxygen anion free radical,hydroxyl free radicals and DPPH radical of reduced hydrolysates were 18 mg /m L,20 mg / m L and 0. 7 mg / m L,respectively. The Fe3 ＋reducing power was 0. 557 when the concentration of peptide was 2 mg / m L. The Hg-bingding mass was 8 mg / g. The antioxidant activities and Hg-binding capacities of reduced soy glycinin hydrolysates were higher than those of non-reduced hydrolysates. It was indicated that reduced soy glycinin hydrolysates possessed potent potential in mercury detoxification.