火棘果多糖抗油脂氧化酸败分析

Analysis for lipid antioxidant activity of polysaccharides from Pyracantha fortuneana

超声波辅助水提火棘果中的多糖,醇沉、分离得到3种多糖PS1、PS2、PS3,以葡萄糖为对照品测定其含量,分析其光谱特征,考察其对油脂的抗氧化酸败水解效果并与VE作比较。结果表明:PS1,PS2,PS3得率分别为0.68%、0.82%、1.25%,3种多糖均为吡喃糖链构型。当质量浓度均为0.3g/L时,PS3对各种油脂的抗氧化活性最好,对菜籽油、橄榄油、鹅油、鸡油、牛油、猪油的抗氧化保护率分别为69.38%、59.19%、56.55%、66.52%、53.72%、66.42%,酸价降低值分别为0.96、0.89、0.84、0.95、0.81、0.91。一定质量浓度范围内,3种多糖对各类油脂的抗氧化酸败水解能力均与其浓度呈现剂量效应关系。

The polysaccharides were extracted from Pyracantha fortuneana with ultrasonic-assisted. The product were precipitated with ethanol,then PS1、PS2、PS3 were obtained,respectively. The content of extracts were determined using glucose as index. The polysaccharides were analyzed with spectrum. The inhibitory effect to oxidation rancidity of oils and fats were investigated,and compared with VE. The results showed that the yield rate of PS1,PS2,PS3 were 0. 68%,0. 82%,1. 25%,respectively. Spectrum analysis showed that these polysaccharides were pyranose chain configuration. When the polysaccharides concentration was 0. 3g / L,the antioxidant activity of PS3 was prominent than others. The protection rates of PS3 for rapeseed oil,olive oil,goose grease,chicken grease,beef tallow,lard fat were 69. 38%,59. 19%,56. 55%,66. 52%,53. 72% and 66. 42%,respectively. The acid value reduction for rapeseed oil,olive oil,goose grease,chicken grease,beef tallow,lard fat were 0. 96,0. 89,0. 84,0. 95,0. 81 and 0. 91,respectively. The results showed that these polysaccharides exhibited excellent ability to protect oils and fats. Furthermore,the antioxidant activity increased with the increasing of the concentration of polysaccharides.