摘要
采取心包积液-肝坏死综合征病鸡的肝脏匀浆冻融,接种SPF鸡胚,制备尿囊液灭活水苗,用两种免疫程序对产蛋鸡进行免疫,用琼脂扩散试验检测其卵黄抗体水平,制备高免卵黄抗体。经中和试验和临床试验,结果显示高免卵黄抗体对该病的预防和治疗效果明显。
In this study, the liver homogenate from the sick chicken with pericardial effusion-hepatic necrosis syndrome was inoculated to SPF chicken embryo to prepare allantoic fluid inactivated vaccine after three times freezing and thawing. Two procedures were employed to immune the hens. Then, agar gel precipitation（AGP） was used to test the antibody level in yolk and prepared the yolk antibody. The neutralization test and clinical test identified that yolk antibody showed an obvious effect on preventing and curing for the disease.
出处
《湖北农业科学》
2016年第8期2053-2054,2057,共3页
Hubei Agricultural Sciences
基金
河南省重点科技攻关项目(152102110089)
河南牧业经济学院科技创新团队项目(HUAHE2015003)
关键词
心包积液-肝坏死综合征
组织灭活水苗
卵黄抗体
回归试验
中和试验
pericardial effusion-hepatic necrosis syndrome
inactivated vaccine
yolk antibody
animal test
neutralization test
