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根癌农杆菌介导水稻成熟胚及抗褐飞虱基因植株的获得

Agrobacterium-mediated Transformation of Rice Mature Embryos and Regeneration of Transgenic Plants with Brown Planthopper(Nilaparvata lugens St覽l) Resistance Genes
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摘要 以水稻(Oryza sativa L.)籼型两系恢复系M5274和晚粳稻不育系N55S成熟胚为材料,以携带有双元载体的根癌农杆菌(Agrobacterium tumefaciens)EHA105为载体进行抗飞虱基因Bph14、Bphi008A、Osg1的遗传转化,共获得515棵再生植株,包括198株Bph14转基因植株、72株Bphi008A转基因植株和245株Osg1转基因植株。PCR检测结果表明,获得的515株再生植株中,有244株阳性转基因植株,3个不同抗褐飞虱基因中,转Bph14基因的再生苗阳性率最高,增加筛选次数能有效减少转化所得的假阳性植株。 The mature embryos of an indica restorer line M5274 and late japonica CMS line N55 S of rice（Oryza sativa L.）were used to study on Agrobacterium-mediated transformation for brown planthopper resistance genes, Bph14、Bphi008A and Osg1. 515 regenerated plants were obtained, including 198 Bph14 transgenic plants, 72 Bphi008 A transgenic plants and 245Osg1 transgenic plants. Through PCR analysis on 515 transgenic plants, 244 of them were found to be positive. Transgenic plants with Bph14 gene had a much higher positive ratio than those plants with Bphi008 A or Osg1 gene. This study also indicated that increasing screen time can availably decrease the negative plants in transformation.
作者 李三和 韩光明 闸雯俊 刘凯 胡刚 游艾青
机构地区 湖北省农业科学院/主要粮食作物产业化湖北省协同创新中心/湖北省作物种质与遗传改良重点实验室
出处 《湖北农业科学》 2016年第9期2392-2395,共4页 Hubei Agricultural Sciences
基金 国家转基因重大专项(2014ZX0800101B) "973"计划项目(2013CBA01405)
关键词 水稻(Oryza sativa L.) 根癌农杆菌(Agrobacterium tumefaciens)介导转化 抗褐飞虱基因 rice（Oryza sativa L ） agrobacterium tumefaciens-mediated transformation brown planthopper resistance genes
分类号 S511 [农业科学—作物学]
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参考文献16

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湖北农业科学
2016年 第9期

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