高脂饲料喂养的GK大鼠血糖水平与饮水量的相关性分析

Analysis of Correlation between Blood Glucose and Water Intake in GK Rats Fed High-fat Diet

目的:观察中、西药干预分别对糖尿病大鼠血糖、饮水量、体质量和摄食量的影响,同时分析糖尿病多饮症状与血糖水平的相关性。方法:自发性2型糖尿病GK大鼠50只,随机分为5组:模型组(M)、参芪复方高剂量组(SH)、参芪复方中剂量组(SM)、参芪复方低剂量组(SL)、格华止组(G)。连续12 w以高脂饲料喂养,诱导并维持高血糖状态。另设正常Wistar大鼠10只,作为空白组(C),予普通饲料喂养,平行对照。12 w后进行药物干预,SH、SM、SL组分别予高剂量、中剂量、低剂量参芪复方汤剂,G组予格华止,C组及M组予生理盐水灌胃。干预疗程16 w。监测大鼠饮水量、摄食量、体质量和空腹血糖,并对饮水量和血糖值进行相关分析。结果:随病程延长,GK大鼠逐渐出现血糖、体质量、摄食量、饮水量不断升高,干预16 w后,SH、SM、SL、G组动物饮水量、摄食量和血糖水平均较M组显著下降(P<0.05);从数值上可以发现,中药各组改善多饮的程度高于G组,而G组大鼠的血糖水平低于各中药组。饮水量与血糖水平呈较弱正相关的关系(r=0.399,P=0.000)。结论:口渴和血糖升高都是糖尿病的客观表现,分别反应同一疾病的不同侧面,血糖并不是糖尿病最核心的指标,也不应作为唯一的控制目标。中、西医由于理论体系的不同,在治疗时各有侧重。

Objective： This study aimed to observation the effect of blood glucose,water intake,body mass and food intake in GK rats respectively intervented with TCM and western medicine,simultaneously investigate the correlation between blood glucose and water intake. Methods： 50 GK rats were randomly divided into 5 groups： model group（ M）,ginseng astragalus compound high dose group（ SH）,ginseng astragalus compound middle dose group（ SM）,ginseng astragalus compound low dose group（ SL）,glucophage group（ G）,induced by high-fat diet for 12 weeks to maintain hyperglycemia. 10 normal Wistar rats were set as the blank group（ C）,which were fed with normal diet. 12 weeks later,SH,SM and SL group were treated with high,middle and low dose Shenqi compound extract gavage,G group treated with glucophage suspension gavage,groups C and M were treated with saline. Body mass,water intake,food intake and fasting blood glucose（ FPG） were monitored every week during the drug intervention. Then analysis the correlation of blood glucose and water intake. Results： With the extension of the course,body mass,water intake,food intake and FPG were all rising. After 16 weeks of treatment,water intake,food intake,and FPG declined in group SH,SM,SL,and G compared with group M. From the numerical,TCM groups to improve polydipsia more than G group,but blood glucose level of G froup was lower than TCM goups. There is a weak positive correlation relationship between water intake and the level of blood glucose. Conclusions： Thirsty and blood glucose are both objective performance of diabetes,reacting different sides of the same disease respectively. Blood glucose is not the core indicators of diabetes,also should not be the only control target. TCM and western medicion each have focus during treatment,because of the difference of theory system.