益阴通络方对局灶性脑梗死大鼠TNF-α、IL-6表达的影响

Effect of TNF- alpha and IL-6 Expression of Yiyin Tongluo Decoction on Focal Cerebral Infarction in Rats

目的:通过动物实验观察益阴通络方对局灶性脑梗死大鼠的疗效,探讨该药治疗缺血性脑血管病的机制。方法:将SD大鼠随机分为假手术组、模型对照组、益阴通络方大剂量组、益阴通络方小剂量组、脑心通组5组,预给药5 d后,除假手术组外,其余各组采用线栓法制作右侧大脑中动脉阻塞模型(MCAO),观察益阴通络方对局灶性脑梗死大鼠神经功能评分及应用免疫组化法检测TNF-α、IL-6的表达。结果:1、MCAO术后,模型组、脑心通组和益阴通络方组均出现不同程度的神经功能障碍,益阴通络方组的神经功能评分明显降低,与模型组、脑心通组相比,益阴通络方大剂量组改善大鼠神经缺失体征优于脑心通组(P<0.05)。2、MCA0术后,模型组、脑心通组和益阴通络方组脑组织内TNF-α和IL-6的表达均增加,与模型组相比、益阴通络方组TNF-α和IL-6的表达均减少,大剂量组有显著统计学差异(P<0.01),小剂量组有统计学差异(P<0.05)。结论:益阴通络方可以降低MCA0术后大鼠的神经功能评分,促进肢体功能的恢复;可能通过调降低炎性细胞因子及抑制病理产物,减少神经元的损伤、从而抑制神经细胞调亡,保护脑细胞。

Objective： Through animal experiment observation of efficacy of Nourishing Yin Recipe after focal cerebral ischemia in rats,of the drug in the treatment of ischemic cerebrovascular disease mechanisms. Methods： SD rats were randomly divided into sham operation group,model control group,Yiyin Decoction high dose group,Yiyin Decoction low dose group,Naoxintong group 5group,pretreatment for 5 days,except the sham operation group,the other groups by thread embolism method making right middle cerebral artery occlusion（ MCAO） model and observation of Nourishing Yin Recipe in local foci of infarction rats neurological function score and application of immune group method to detect TNF-a,IL-6 expression. Results： 1. After MCAO,model group,cerebral Xintong group and nourishing Yin Recipe group appeared different degree of neurological dysfunction,Yiyin Tongluo Decoction group,the nerve function score was significantly lower,compared with the model group,Naoxintong group,Yiyin Tongluo Decoction high dose group improved rat neural missing signs Naoxintong group was superior to the（ P〈0. 05）. 2. MCA0 after operation,the model group,brain Xintong group and Yiyin Tongluo Decoction group brain tissue TNF alpha and IL-6 expression were increased. Compared with the model group,Yiyin Tongluo Decoction group of TNF-a and IL-6 expression were reduced,large dose group has significant statistical difference（ P〈0. 01）,small dose group had statistical difference（ P〈0. 05）. Conclusion： Nourishing Yin Recipe can reduce the postoperative MCA0 rats of the nerve function score,promote the recovery of limb function,by lowering the low inflammatory cytokines and inhibit the pathological product,reduce neuronal damage,thereby inhibiting nerve cell apoptosis,protect brain cells.