石菖蒲煎剂对不同时长急性脑缺血再灌注大鼠BBB保护效应的研究

Study on Protective Effect of Water Decoction of Rhizoma Acori Tatarinowii on Blood Brain Barrier in Rats with Different Time of Acute Cerebral Ischemia Reperfusion

目的:证实石菖蒲煎剂对血脑屏障有保护作用,研究石菖蒲煎剂对不同时长急性脑缺血再灌注大鼠血脑屏障(Blood Brain Barrier BBB)保护效应,并探索石菖蒲煎剂治疗延长脑缺血溶栓时间窗的可能性。方法:将96只成年雄性SD大鼠随机分为4组,即假手术组、模型组、单纯再灌组、石菖蒲再灌组,每组24只;各组随机分为MCAO3h,MCAO6h 2个亚组,每组12只;复制大鼠大脑中动脉缺血模型MCAO,假手术组行假手术。在溶栓后24 h,每个亚组随机分成两组,每组6只,分别用荧光法测定大鼠脑内伊文斯蓝(EB)的含量以及大鼠脑含水量和脑系数。结果:1MCAO3h模型,与单纯再灌组比较,石菖蒲再灌组脑含水量、脑系数、脑EB含量无明显差异(P>0.05);与假手术组比较,石菖蒲再灌组与脑含水量无明显差异(P>0.05),脑系数提高(P<0.05);2MCAO6h模型,与单纯再灌组比较,石菖蒲灌再灌组脑含水量、脑系数及脑EB含量均下降(P<0.05);与模型组比较,石菖蒲再灌组脑含水量及脑系数明显下降(P<0.05),单纯再灌组脑EB含量升高(P<0.05);3与3 h石菖蒲再灌组比较,6 h石菖蒲再灌组脑EB含量升高(P<0.05)。结论:石菖蒲煎剂对血脑屏障有保护作用,能改善其通透性,降低缺血再灌注损伤;在一定时间内,脑缺血时间越长石菖蒲煎剂对血脑屏障的保护作用越明显;石菖蒲水煎剂具有延长大鼠溶栓治疗时间窗的可能性。

Objective： To confirm the protection of the water decoction of rhizoma acori tatarinowii on blood brain barrier, study the protective effect of water decoction of rhizoma acori tatarinowii on blood brain barrier in rats with different time of acute cere-bral ischemia reperfusion, and explore whether the water decoction of rhizoma acori tatarinowii could prolong thrombolytic time window of cerebral ischemia. Methods： 96 SD adult male rats were randomly divided into 4 groups named sham operation group, model group, pure reperfusion group and rhizoma acori tatarinowii reperfusion group. Every group had 24 rats. Then every group was randomly divided into two subgroups called MCAO3h and MCAO6h. Every subgroup had 12 rats. The middle cerebral artery occlusion models were established in rats. And the sham operation group received sham surgery. At 24 h after thrombolysis, every subgroup were random- ly divided into 2 groups, and the contents of Evans blue （EB）, brain water and cerebral coefficient in rats were determined by fluo- rescence spectroscopy. Resets ： （ 1 ） In MCAO3h models, compared with pure reperfusion group and rhizoma acori tatarinowii reper- fusion group, the content of brain water, cerebral coefficient and the content of EB had no obvious difference （P 〉 0. 05 ）. Compared with sham operation group, rhizoma acori tatarinowii reperfusion group＇s content of brain water had no obvious difference （ P 〉 0. 05 ）. And its＇ cerebral coefficient increased （ P 〈 0. 05 ）. （2） In MCAO6h models, compared with pure reperfusion group and rhizoma acori tatarinowii reperfusion group, the content of brain water, cerebral coefficient and the content of EB all decreased （ P 〈 0. 05 ）. Compared with model group, rhizoma acori tatarinowii reperfusion group＇s content of brain water and cerebral coefficient decreased ob- viously （ P 〈 0. 05 ） . And rhizoma acori tatarinowii reperfusion group＇s content of EB increased （ P 〈 0. 05 ）. （ 3 ） Compared with rhizoma acori tatarinowii reperfusion 3 h group, rhizoma acori tatarinowii reperfusion 6h group＇s content of EB increased （P 〈 0. 05 ）. Conclusion： The water decoction of rhizoma aeori tatarinowii has a protective effect on blood brain barrier, which can improve its per- meability and reduce isehemia-reperfusion injury. And it has a better protective effect on longer time of cerebral ischemia. Also the water decoction of rhizoma acori tatarinowii can prolong thrombolytic time window in rats.