贫营养好氧反硝化菌群的筛选及源水脱氮特性

Isolation of oligotrophic aerobic denitrifiers and its characteristics of nitrogen removal in source water

为了对低浓度氮污染的水体进行净化,通过对微污染水库沉积物进行富集驯化培养,得到好氧反硝化脱氮混合菌。对混合菌进行传代培养得到10^#为效果最好的混合菌。将10#混合菌投加到微污染水库源水中进行投菌实验。实验结果表明：投菌系统的硝氮从初始的（0.38±0.03）mg/L降到（0.07±0.03）mg/L,硝氮的去除率达到（82.30±2.63）%,而空白对照系统的硝氮从初始的（0.34±0.00）mg/L到30 d实验结束变为（0.69±0.01）mg/L;复合菌投菌系统并没有出现亚硝氮的积累;投菌系统总氮从初始的（1.00±0.04）mg/L,到30 d实验结束时总氮下降到（0.52±0.03）mg/L,去除率达到（48.24±2.82）%,但是空白对照系统的总氮从初始的（1.13±0.06）mg/L到30 d结束时为（1.17±0.03）mg/L,基本保持稳定;复合菌系统的好氧反硝化菌菌落数高于空白系统1～2两个数量级。脱氮效果表明,应用复合好氧反硝化菌对于源水脱氮有很好的应用潜力。

To purify micro-polluted source water,mixed denitrification bacterial species were isolated based on enrichment,domestication,and screening processes. The 10^#mixed denitrification bacteria with the best nitrogen removal performance were used in a source water experiment. Nitrate in the bacterial system decreased from（ 0. 38 ± 0. 03） mg / L to（ 0. 07 ± 0. 03） mg / L,and the nitrate removal rate reached（ 82. 30 ± 2. 63） %.However,nitrate in the blank system increased from（ 0. 34 ± 0. 00） mg / L to（ 0. 69 ± 0. 01） mg / L,and no nitrite accumulation was observed. The total nitrogen（ TN） of the bacterial system decreased from（ 1. 00 ± 0. 04） mg /L to（ 0. 52 ± 0. 03） mg / L,while in the blank system,TN was maintained at（ 1. 13 ± 0. 06） to（ 1. 17 ± 0. 03）mg / L. The numbers of aerobic denitrification bacteria in bacteria system was higher than that in blank system（ 1 to 2 orders of magnitude）. These results suggested that the mixed denitrification bacteria can be used as microbial inoculum for nitrogen removal and have practical applications in the micro-polluted water treatment process.