手性离子液体修饰纳米金粒子用于酪氨酸对映体的手性识别

Application of Chiral Ionic Liquid Modified Gold Nanoparticles in the Chiral Recognition of Tyrosine Enantiomers

本文以手性离子液体1-乙基-3-甲基咪唑L-酒石酸盐(EMIML-Tar)为修饰剂合成了EMIML-Tar修饰的金纳米粒子(EMIML-Tar-AuNPs)。采用透射电镜、紫外-可见分光光度计对EMIML-Tar-AuNPs进行了表征。合成的EMIML-Tar-AuNPs平均粒径约为6.5nm,其紫外-可见吸收光谱的最大吸收波长为520nm。进一步将EMIML-Tar-AuNPs用于酪氨酸(Tyr)对映体的手性识别,观察添加不同浓度D-Tyr和L-Tyr的EMIML-Tar-AuNPs的颜色变化及其紫外-可见吸收光谱。结果表明,经过D-Tyr和L-Tyr处理的EMIML-Tar-AuNPs胶体的颜色不同,而且紫外-可见光谱图也存在明显差异。对各浓度光谱图在650nm和520nm处的吸光度比值进行比较,结果发现各浓度D-Tyr的吸光度比值均较低且差别不大,而L-Tyr的吸光度比值均较高,且随着浓度的增大会出现大幅度增加。

Using the chiral ionic liquid（1-ethyl-3-methylimidazole L-tartrate,EMIML-Tar）as the modifier,EMIML-Tar modified gold nanoparticles（EMIML-Tar-AuNPs）were synthesized.Transmission electron microscope and UV-Vis spectrophotometer were used for the characterization of EMIML-TarAuNPs.The average particle size of the synthesized EMIML-Tar-AuNPs was about 6.5nm,and the maximum absorption wavelength was 520 nm.EMIML-Tar-AuNPs were further applied in the chiral recognition of tyrosine（Tyr）enantiomers.The changes in the color and UV-Vis absorption spectra of EMIML-Tar-AuNPs in the presence of D-Tyr and L-Tyr were observed.The ratios of absorption intensities at 650 nm and 520 nm were compared.It was found that the absorbance ratios of D-Tyr with different concentrations were lower and had no obvious change,while the absorbance ratios of L-Tyr were larger and had a substantial increase with the concentration increasing.