HGF蛋白与C-Met蛋白在胆管癌中的表达及临床意义

Expression and clinical significance of HGF and C-Met in cholangiocarcinoma

目的探讨肝细胞生长因子(HGF)与其受体(C-Met)蛋白在血清及组织中的表达及临床意义。方法采用酶联免疫吸附测定(ELISA)检测42例初治胆管癌患者及20例健康对照者血清HGF及C-Met水平;应用免疫组织化学技术观察42例胆管癌组织及正常组织HGF及C-Met的表达。结果术前1d胆管癌患者血清中HGF蛋白与C-Met蛋白水平均高于健康对照者(1.53±0.48)ng/mL vs.(0.48±0.55)ng/mL;(16.28±5.17)ng/mL vs.(4.31±2.84)ng/mL,差异均有统计学意义(P<0.05)。术后第3天胆管癌患者血清中HGF蛋白与C-Met蛋白水平较术前变化不明显,而术后第7、10天HGF蛋白与C-Met蛋白水平较术前则明显降低。另外,HGF蛋白与C-Met蛋白在胆管癌组织的阳性表达率分别为76.2%、81.0%,在正常组织的表达分别为15.0%、20.0%。结论 HGF蛋白与C-Met蛋白在胆管癌患者血清和组织中异常表达,可能与胆管癌的发生、发展有密切关系。

Objective To investigate the expression and clinical significance of HGF and C-Met in cholangiocarcinoma .Meth-ods The levels of HGF and C-Met were measured by ELISA ,the expression of HGF and C-Met in cholangiocarcinoma and health control was detected by immunohistochemistry .Results The preoperative levels of HGF and C-Met in cholangiocarcinoma were higher than the health control（P〈0 .05） .Compared with the preoperative state ,the levels of HGF and C-Met in cholangiocarcino-ma on the 3rd day after operation did not change significantly .However ,the levels of HGF and C-Met on the postoperative 7th and 10th day decreased obviously .In addition ,the expression of HGF and C-Met in cholangiocarcinoma was 76 .2% ,81 .0% ,respective-ly ,which is higher than that in normal tissue （ P 〈0 .01） .Conclusion There is an abnormal expression of HGF and C-Met in cholangiocarcinoma ,which may be related to the incidence and development of cholangiocarcinoma .