摘要
1目的预测黑色素瘤相关抗原-4(MAGE-4)抗原的MHC-Ⅰ/MHC-Ⅱ限制性表位肽。2方法利用NCBI蛋白数据库检索到MAGE-4抗原的氨基酸序列,BIMAS、SYFPEITH1、IEDB等3种方案分别对MAGE-4抗原的HLA-A*0201限制性MHC-Ⅰ类抗原表位进行预测,选出候选肽;然后利用SYFPEITHI服务器和NetMHCIIpan 3.1服务器对候选肽周围的MHC-Ⅱ限制性表位进行预测。综合分析选出MHC-Ⅰ/MHC-Ⅱ限制性表位肽。3结果筛选得到3条肽段,既存在MHC-Ⅰ限制性表位又存在MHC-Ⅱ限制性表位肽。4结论为MAGE-4抗原用于结肠癌免疫治疗肽段的选择提供了依据。
Objective To predict the MHC-Ⅰ/MHC-Ⅱ restricted epitope peptide of MAGE-4.Methods The amino acid of MAGE-4was retrieved by NCBI protein datebase.Based on this,HLAA＊0201restricted epitope peptide was predicted by BIMAS、SYFPEITH1and IEDB on line service.Then we used SYFPEITHI and NetMHCIIpan 3.1to predicted MHC-Ⅱ restricted peptide.ResultsThree peptides were obtained,which contain MHC-Ⅰ restricted epitope as well as MHC-Ⅱ restricted epitope.Conclusion This result provide a basis for choosing peptides in order to immunotherapy of colorectal cancer.
出处
《河北联合大学学报（医学版）》
2016年第3期174-178,共5页
Journal of North China Coal Medical College
