聚季铵盐离子液体材料制备及其对蛋白质吸附性能评价

Preparation of poly (quaternary ammonium ionic liquid)s materials and their adsorption properties for proteins

通过一步合成法制备了两种可聚合季铵盐离子液体功能单体,并通过沉淀聚合法合成了相应的聚离子液体聚合物。对产物进行了核磁共振、扫描电镜、热重分析等表征。结果表明:所制备的两种材料粒径均匀,约为600nm的椭球形颗粒,颗粒之间有相互粘连。通过对牛血清白蛋白(BSA)、卵清蛋白(OVA)、牛血红蛋白(BHb)、溶菌酶(Lys)、胰蛋白酶(Try)5种蛋白质的吸附性能实验,考察了聚季铵盐离子液体材料对蛋白质的吸附性能。考察结果表明:两种聚离子液体材料均对蛋白质具有一定的吸附性能。其中以4-乙烯基苄氯季铵盐离子液体为功能单体制备的聚离子液体材料对胰蛋白酶的吸附性能最好,是一种具有良好应用前景的材料。

The ionic liquids as a new class of green solvent have received the majority attention. In this study,two polymeric ionic liquid monomers were synthesized by one step method,and polymerized by precipitation polymerization method. The resultant materials were characterized by nuclear magnetic resonance spectroscopy（1H NMR）,scanning electron microscopy（SEM）and thermo gravimetric analyzer（TGA）methods. The results showed that the morphology of the resultant materials was ellipsoidal particles with the size of about 600 nm. The adsorption properties of the resultant materials for bovine serum albumin（BSA）,ovalbumin（OVA）,bovine hemoglobin（BHb）,lysozyme（Lys）and trypsin（Try）were also evaluated.The two poly（ionic liquid）s materials showed excellent adsorption properties for proteins. The poly（4-vinyl benzyl chloride quaternary ammonium ionic liquid）s material exhibited the best adsorption to trypsin. Such materials show potential in biomedical and material engineering.