炎性肿块肺纤维化组织中miR-21和TGFβ1的表达变化

Expression of MiR-21 and TGFβl in Pulmonary Fibrosis of Inflammatory Mass and Its Signifi-cance

【目的】观察炎性肿块肺纤维化组织中miR-21和转化生长因子B1（transforminggrowthfactor171，TGFβ1）的表达变化，探讨两者的表达变化是否与肺纤维化形成有关。【方法】选择10例炎性肺部肿块标本，每例分别按纤维化组织和旁近正常肺组织配对留取，所有标本均采用Real-timePCR方法检测组织中miR-21和TGFβ1的表达量变化，分析其表达是否与肺纤维化有关。【结果】肺纤维化组织中miR-21和TGFβ1的表达明显高于正常旁近肺组织，且两者相比较差异均有显著性（P〈0．01）。【结论1miR-21和TGFβ1在炎性肿块肺纤维化组织中高表达，其高表达与炎症导致的肺纤维化形成有关。

[Objective]To examine the levels of expression of microRNA-21 （miR-21） and transforming growth factorβ1 （TGF-β1）in lung tissues with fibrosis and its adjacent non fibrosis tissues.[Methods]Specimens of pulmonary fibrosis tissue and adjacent tissues were collected from 10 patients. Their miR-21 and TGFβ1 expression levels were detected by Real-time PCR. [Results]High sensitivity, specificity, and precision of RT-PCR allowed for direct analy- sis of expression of miR-21 and TGFβ1 expression in pulmonary fibrosis tissues and adjacent tissues. The expression miR-21 and TGFβ1 in pulmonary tissues was significantly higher than in adjacent tissues （ P〈0.001）. [Conclusions] Elevated expression of miR-21 and TGFβ1 play an important role in the pathogenesis of diseases related to fibro-genic reactions in pulmonary inflammatory mass patients.