电针大肠俞募穴对功能性便秘小鼠胃肠传输功能的影响

Effect of electro-acupuncture at Back-shu point and Frontmu point on gastrointestinal function in mice with functional constipation

目的:探讨电针大肠俞募穴对功能性便秘小鼠胃肠传输功能的影响.方法:56只小鼠随机分为空白对照组、模型组、电针组、药物组、肠神经胶质细胞(enteric glial cell,EGC)对照组、EGC针刺组、对照针刺组.采用复方地芬诺酯灌胃造模,电针刺激"天枢"、"大肠俞"、药物组采用西沙比利灌胃,1次/d,5 d一个疗程,疗程之间休息2 d,共治疗2个疗程.治疗结束后观察胃排空、小肠推进率、肠道通透性及结肠平滑肌收缩张力变化情况.结果:与空白对照组比较,模型组小鼠胃排空率、小肠推进率减慢(P<0.01,P<0.01),肠道通透性增加(P<0.01),结肠平滑肌收缩张力呈下降趋势;与模型组相比,针刺组和药物组小鼠胃排空率、小肠推进率增强(P<0.01,P<0.01),肠道通透性降低(P<0.05),针刺组结肠平滑肌收缩张力增强(P<0.05);与针刺组比较,EGC对照组、EGC针刺组小鼠胃排空率减慢(P<0.05,P<0.05),小肠推进率降低(P<0.01,P<0.01),而药物组各指标变化不明显;与EGC对照组比较,EGC针刺组各指标变化不明显;与空白对照组比较,对照针刺组小鼠针刺后各指标变化不明显.结论:在健康状态下,电针俞募穴对胃肠传输功能的作用不明显;在疾病状态下,电针俞募穴可能通过调节EGC的功能进而改善功能性便秘小鼠的胃肠传输功能.

AIM：To explore the effect of electro-acupuncture（EA） at Back-shu point and Front-mu point on gastrointestinal function in functional constipation（FC） mice.METHODS：Fifty-six mice were randomly divided into the following seven groups：blank control,model,EA,drug,EGC control,EGC acupuncture,and blank acupuncture.A mouse model of FC was developed by compound diphenoxylate lavage.After modelling,three of the groups underwent EA stimulation at points ＂Tianshu（ST25）＂ and ＂Dachangshu（BL25）＂ with one of the groups received cisapride by gavage once a day.Five treatments comprised a course and there was a 2-day interval between two courses.Gastric emptying,small intestinal transit rate,intestinal permeability,as well as the amplitude of the colon contraction were assessed after two courses of treatment.RESULTS：Gastric emptying and small intestinal transit rate in the model group were significantly reduced（P 〈0.01）,but intestinal permeability was increased（P 〈0.01）.EA at Back-shu point and Front-mu point can improve gastric emptying and small intestinal transit rate（P 〈0.01,P 〈0.01）,reduce intestinal permeability（P 〈0.05） and increase the amplitude of the colon contraction（P 〈0.05）.Compared with the EA group,gastric emptying and small intestinal transit rate were reduced in the EGC control group（P 〈0.05,P 〈0.01） and EGC acupuncture group（P 〈0.05,P 〈0.01）,but no significant differences were observed in the drug group.Compared with the EGC control group,There were also no significant differences between the EGC control group and EGC acupuncture group.The change of each index was not obvious in the blank acupuncture group.CONCLUSION：Electro-acupuncture at Backshu point and Front-mu point has no significant impact on gastrointestinal function in normal mice;however,it may improve gastrointestinal function by regulating the function of EGC cells in FC mice.