皮肤软组织感染的病原菌分布与耐药性分析

Bacterial distribution and resistance in skin and soft tissue infections

目的了解皮肤软组织感染细菌的分布及耐药状况。方法收集2010—2014年北京大学第一医院门诊和住院患者的皮肤软组织感染标本,常规方法进行细菌培养和鉴定,按纸片法、微量稀释法或E-test法测定细菌药物敏感性。结果共分离出非重复细菌1793株,排在前4位的依次是金黄色葡萄球菌、表皮葡萄球菌、大肠埃希菌和铜绿假单胞菌,分别占32.6%,10.7%,10.7%,10.3%。甲氧西林敏感金黄色葡萄球菌(MSSA)分离率多年来一直居首位。未检出万古霉素耐药金黄色葡萄球菌和表皮葡萄球菌,对利奈唑胺耐药率分别为0.2%和0。大肠埃希菌和铜绿假单胞菌对亚胺培南的耐药率分别为0和13.6%。结论皮肤软组织感染的主要致病菌仍以金黄色葡萄球菌为主。

Objective To investigate the bacterial distribution and resistance to antibiotic in skin and soft tissue infections. Methods All the stains of outpatients and inpatients were isolated from infectious skin and soft tissue in the last 5 years. The stains were cultured and identified by conventional method,and their susceptibility were tested by disk diffusion,microbroth dilution or E- test according to Clinical and Laboratory Standard Institute（ CLSI,2015） manual. Results A total of 1793 strains were collected. The four main species were Staphylococcus aureus,Staphylococcus epidermidis,Escherichia coli and Pseudomonas aeruginosa,accounting for 32. 6%,10. 7%,10. 7% and 10. 3% respectively.The methicillin sensitive Staphylococcus aureus（ MSSA） isolation rate has been in the first place for many years. There was no resistance to vancomycin for Staphylococcus aureus and Staphylococcus epidermidis. And their proportions of resistant to linezolid were 0. 2% and 0 respectively. The proportions of Escherichia coli and Pseudomonas aeruginosa strains resistant to imipenem were 0 and 13. 6% respectively. Conclusion Staphylococcus aureus was predominant organism in skin and soft tissue infections.