常春藤皂苷元对肠癌SW480细胞上皮-间质转化(EMT)及侵袭的影响

Effects of Hederagenin in Suppressing Epithelial-mesenchymal Transition and Invasion of Colon Cancer Cells SW480

目的:探讨常春藤皂苷元(HG)通过调控上皮-间质转化(EMT)途径抑制大肠癌侵袭转移的机制。方法:以肠癌细胞SW480细胞为研究对象,不同质量浓度HG干预转化生长因子-β1(TGF-β1)诱导的SW480细胞,作用24 h后噻唑蓝(MTT)法检测细胞增殖能力。转移小室(Transwell)实验检测细胞侵袭能力。实时荧光定量-聚合酶链式反应(q PCR)和蛋白质免疫印迹(Western blot)法分别检测EMT标志物E-钙黏蛋白(E-cadherin),N-钙黏蛋白(N-cadherin),波形蛋白(Vimentin),蜗牛同源物1(果蝇)样1蛋白(Snail)及侵袭转移标志分子信号传导蛋白和转录激活物(STAT3),基质金属蛋白酶-9(MMP-9),MMP^(-1)4,伴有Kazal域的富含半胱氨酸的逆转诱导蛋白(RECK)表达变化。结果:HG可抑制TGF-β1诱导的肠癌SW480细胞增殖、干预EMT和降低侵袭能力,降低EMT标志分子N-cadherin,Vimentin,Snail及侵袭转移标志分子STAT3,MMP-9,MMP^(-1)4表达,增强E-cadherin,RECK的表达,与TGF-β1组比较均具有统计学差异(P<0.05)。结论:HG可通过抑制EMT途径影响大肠癌细胞SW480侵袭转移。

Objective： To investigate whether hederagenin（ HG） could inhibit the invasion and metastasis of colorectal cancer by epithelial-mesenchymal transition（ EMT） pathway. Method： MTT was used to determine the proliferation of transforming growth factor（ TGF）-β1-induced colorectal cancer cells SW480 with different doses of HG treating for 24 h. Transwell assay was used to determine the inhibitory effects of HG on cell invasion in SW480 cells. Western blot and real-time fluorescent quantitative polymerase chain reaction（ q PCR）assay were used to observe the expressions of EMT markers such as E-cadherin,N-cadherin,Vimentin,Snail,and invasion and metastasis markers such as signal transducer and activator of transcription（ STAT）-3, matrix metalloproteinase（ MMP）-9,reversion-inducing cysteine-rich protein with Kazal motif（ RECK） and MMP-14.Result： Here we found that HG inhibited the proliferation of SW480 cells which induced by TGF-β1,influenced EMT and reduced the invasion ability. Furthermore,HG reduced the expressions of EMT markers including Ncadherin,Vimentin,Snail and invasion and metastasis markers including STAT-3,MMP-9,MMP-14,enhanced the expressions of E-cadherin and RECK（ P〈0. 05）. Conclusion： HG inhibited the invasion and metastasis of SW480 cells by influencing EMT pathway.