ARVCF和Rac1在非小细胞肺癌组织中的表达及意义

Expression and significance of ARVCF and Rac1 in non-small cell lung cancer

目的探讨在非小细胞肺癌组织中ARVCF与Rac1的表达相关性。方法免疫组化法检测130例非小细胞肺癌标本中ARVCF和Rac1的表达水平。采用Western Blotting法检测30例新鲜肺癌组织及周围正常肺组织中ARVCF和Rac1蛋白表达量。并在ARVCF表达较高的A549细胞系中干扰ARVCF表达后,检测Rac1的蛋白表达及活性的改变。结果 ARVCF和Rac1在肺癌组织表达水平均明显高于正常肺组织,二者呈正相关。ARVCF和Rac1的共表达与具有高临床分期,低分化,腺癌,淋巴结转移等临床病理因素呈正相关,共表达的肺癌患者具有更短的生存时间。在A549细胞系中下调ARVCF后,Rac1的表达和活性均降低,并减弱肺癌细胞的侵袭能力。结论 ARVCF和Rac1在非小细胞肺癌中共表达,与患者的不良预后正相关,而ARVCF通过调节Rac1表达与活性影响肺癌细胞侵袭能力。

Objective To investigate the relationship between ARVCF and Rac1 expressions in non-small cell lung cancer. Methods We examined the expression of ARVCF and Rac1 by Immunohistochemistry in 130 cases of nonsmall cell lung cancer and Western Blotting in 30 cases of fresh lung cancer tissues and normal lung tissues. After downregulating ARVCF in A549 cell line which expressed a relatively higher level of ARVCF, the expression and activity of Rac1 were detected. Results The expression levels of ARVCF and Rac1 were significantly higher in lung cancer tissue than in normal lung tissue. The positive expression of ARVCF was positively correlated with the overexpression of Rac1（ 〈0.001）. The co-expression of ARVCF and Rac1 was also positively correlated with clinical pathological factors such as high clinical stage, poor differentiation, adenocarcinoma, lymph node metastasis and so on. The lung cancer patients with the co-expression had a shorter survival time. After knocked down ARVCF in A549 cell line, the expression and activity of Rac1 were decreased,leading to the reduced invasive ability of lung cancer cells. Conclusion The co-expression of ARVCF and Rac1 was positively correlated with poor prognosis of NSCLC patients, ARVCF affacted invasive ability of lung cancer cells by regulating the expression and activity of Rac1.