摘要
目的研究白细胞介素6(IL-6)-174位点和-634位点G/C多态性在辽西地区慢性心力衰竭(CHF)患者的多态性分布及相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法,对80例慢性心力衰竭患者和健康对照组80例受试者,研究IL-6-174和IL-6-634位点基因多态性分布。结果 IL-6-634位点等位基因G、C频率对照组分别为13.13%、86.87%,CHF组分别为23.12%、76.88%,等位基因G在CHF组检出率明显高出对照组(<0.05)。IL-6-174位点等位基因G、C频率对照组为98.13%、1.87%,CHF组为98.75%、1.25%,差异无统计学意义(>0.05)。结论 IL-6-634 G多态性与CHF发病具有相关性,提示等位基因G可能是部分CHF患者易感性的遗传标志。
Objective To study the polymorphism of 174G/C and 634G/C loci in the promoter region of interleukin 6（IL-6） in patients with chronic heart failure. Methods Polymerase chain reaction–restriction fragment length polymorphism technique was applied to analyze the polymorphism of IL-6-174 G/C and IL-6-634 G/C in the chronic heart failure patients（80 cases） and healthy group（80 cases）. Results The frequency of allelic gene G of IL-6-634 G/C loci was higher in CHF group（23.12%） than in healthy group（13.13%, 〈0.05）,but with no difference for C between two groups, also with no difference for IL-6-174 G/C loci between two groups（ 〉0.05）. Conclusion The correlation of IL-6-634 G polymorphism to chronic heart failure indicates that G allele might be a susceptibility genetic marker for CHF patients.
出处
《解剖科学进展》
2016年第3期318-320,共3页
Progress of Anatomical Sciences
基金
国家级大学生创新创业训练计划项目(2014101600 22)
辽宁省大学生创新创业训练计划项目(2014 10160022)
辽宁省教育厅高等学校科学研究一般项目(L2014329
L2014330)
