新疆传统发酵酸驼乳优势乳酸菌粘附性质及益生特性研究

Study on cell adhesion and probiotic characteristics of dominant lactic acid bacteria in traditional fermented camel milk-shubat

以从新疆传统发酵酸驼乳优势乳酸菌中筛选出的4株经初步鉴定能耐酸耐胆盐的乳酸菌菌株为研究对象,选取人体肠上皮细胞株Caco-2,采用显微观察和平板计数的方法研究这4株菌对细胞的粘附性质;采用高效液相色谱法进行体外降胆固醇能力研究;并研究了4株乳酸菌对指示菌的抑菌能力。结果显示,Le2-1（Leuconostoc lactis strain）、M4-3-2（Enterococcus sp.）、M3-1（Enterococcus faecium strain）、M3-3（Lactobacillus brevis strain）都具有较强的粘附能力,其中M3-1的粘附能力最强。4种菌株体外胆固醇降解率在28%~41%（M4-3-2的降解率达到40%以上）,并且对指示菌都具有较强的抑菌活性。

Adopting alternative culture 4bacteria trains among the superior bacteria were screened from Xinjiang camel acid-milk.Identified as lactic acid bacteria,and own high ability of resistance to acid and salt.The adhensive ability of the strains to cell line Caco-2was tested via morphological observation and cell counting under microscope.The amount of cholesterol assimilated by the bacteria was measured by high performance liquid chromatography（HPLC）.As results,Le2-1（Leuconostoc lactis strain）, M4-3-2（Enterococcus sp.）,M3-1（Enterococcus faecium strain）,M3-3（Lactobacillus brevis strain）were adhensive to Caco-2.M3-1was the most adhesive strain among the four tested strains.The cholesterol assimilated range from 28%~41%,M4-3-2which Assimilated more than 40%Cholesterol from culture medium.The antibacterial capacities of4 strains were studied.The result showed that the metabolins of4 strains were of obvious antibacterial activity.