含银杏叶提取物胶囊中黄酮类化合物检测方法改进

Improvement of determination method of total flavonoids in extract of Ginkgo bilobaleaves of capsules

黄酮类化合物是银杏叶提取物中最重要的成分之一,采用亚硝酸钠—硝酸铝—氢氧化钠显色法测定保健食品中总黄酮含量存在一定缺陷。通过研究6种银杏叶提取物中主要的黄酮类组分对总黄酮含量的贡献值,结果表明槲皮苷是该方法下对总黄酮含量贡献最大的组分。进一步研究了该方法测定HZ胶囊（某公司生产的银杏叶提取物胶囊）中总黄酮含量过程中取样量、洗脱液乙醇浓度和超声时间对测定结果的影响,结果表明洗脱液乙醇浓度和超声时间对HZ胶囊中总黄酮含量具有重要影响。改进方法后,HZ胶囊中总黄酮含量在1.68~1.83g/100g,平均含量为1.71~1.77g/100g,相对标准偏差为1.36%~4.08%,芦丁回收率为94.05%~100.44%,重复性、精密度和加标回收率结果表明改进后的方法符合HZ胶囊生产过程用于质量控制的检测方法要求。

Flavonoids are the pharmacologically important chemical groups.NaNO2-Al（NO3）3-NaOH colorimetry assay for determination of total flavonoids in health foods have some defects.In this work,contribution of six key flavonoids components of extract of Ginkgo biloba leaves to total flavonoids content in the NaNO2-Al（NO3）3-NaOH colorimetry assay were evaluated.Results showed that quercitin made the most contribution to total flavonoids content.Sample quantity,alcohol concentration of eluant,and ultrasonic times in this assay were evaluated.Alcohol concentration of eluant and ultrasonic times affected total flavonoids content in HZ Capsules.By using the improved method,total flavonoids content of HZ Capsules were between 1.68g/100 g and 1.83g/100 g,the average contents ranged from 1.71g/100 g to 1.77g/100 g,the relative standard deviation were between 1.36%and 4.08%,the recoveries of rutin ranged from 94.05% to 100.44%.Results from the repetitive test,precsiion test and recovery test indicated that this improved method could meet the demand for determination of total flavonoids in HZ Capsules to quality control during processing.