超高压提取蛹虫草鲜汁中虫草素工艺优化

Optimizationon improving dissolved quantity of cordycepin from Cordyceps militari juice by ultra-high pressure processing

为了优化超高压提取蛹虫草汁中虫草素溶出量工艺,以压力、时间、料液比和循环次数为自变量,虫草素溶出量为响应值,进行响应面试验设计与优化,并建立预测数学模型。结果表明:虫草素超高压提取的最佳工艺为压力418 MPa、时间33min、料液比1:2(m:V)、循环1次,该条件下虫草素溶出量为(84.69±1.65)mg/g·提取物,与预测值(84.03mg/g·提取物)的相对标准偏差为0.61%,获得的数学模型能够用于预测超高压提高蛹虫草汁中虫草素溶出量的过程。

In order to study the ultra-high pressure extraction of cordycepin fromCordyceps militaris fresh juice,the effect of opration conditions such as pressure,time,solid-liquid ratio and cycle number were analyzed.On the basis of single-factor test,the optimum extraction technology of cordycepin and mathematical regression model were established by response surface methodology.The result showed that the optimal extraction conditions were as followed：extraction pressure of 418 MPa,extraction time of 33 min,solid-liquid ratio of 1：2（m：V）,cycle number of 1.Under these conditions,the measured value of dissolved quantity of cordycepin was 84.69mg/g of extract,to which the relative standard deviation of predicted value（84.03mg/g of extract）was 0.61%.The standardized regression equation can be used to predict the extraction yield of cordycepin fromC.militaris fresh juice.