可利霉素的三级逆流萃取纯化

Purification of Kelimycin with Three-stage Counter Current Extraction

杂质依据极性的不同可分为强极性、中等极性和弱极性3类,在不同溶剂中的溶解性能有显著差异。本研究应用三级逆流离心萃取技术纯化可利霉素,考察洗涤条件对不同杂质的去除情况。结果显示,洗涤步骤对3类杂质均有去除效果,但对较强极性杂质的去除更有效。优化洗涤条件,以1%磷酸二氢钠溶液作为洗涤液,在20℃、水酯相比1∶1.5、离心萃取器相对离心力为74×g时,总杂质分离效果较好。除杂率大于50%,较传统的错流萃取工艺提高15%。

Impurities could be divided into three groups according to their polarities： strong, medium and weak polarity. They have different solubility in distinct solvents. A three-stage counter current centrifugal extraction method was used for the puriftcation of kelimycin. The effects of scrubbing on the removal of different impurities were also investigated. The results showed that the impurities with different polarities could be reduced after the scrubbing process, however, the removal effect on the strong polar impurities was more obvious. After optimization, the conditions were determined as follows： temperature of 20 ℃, the aqueous/ester phase ratio of 1： 1.5, RCF of 74×g and the concentration of sodium dihydrogen phosphate of 1%. The removal rate for the total impurities could be over 50%, which was a 15 % increase compared with the traditional cross flow extraction process.