减肥胶囊中维生素BT的毛细管电泳法测定

Determination of Vitamin BT in Slimming Capsules by Capillary Electrophoresis

采用毛细管电泳-间接紫外法测定减肥胶囊中的维生素BT。以4 mmol/L奎宁甲醇溶液作为背景吸收物质,以20mmol/L磷酸二氢钠溶液（p H 2.7）作为运行缓冲溶液,运行电压10 k V,检测波长218 nm。维生素BT在1~40μg/ml范围内线性关系良好,回收率为99.6%,RSD为1.9%。

A capillary electrophoresis method was established for the determination of vitamin BT in slimming capsules with indirect UV detection. The method was carried out with 4 mmol/L quinine methanol solution as the absorbable matrix, 20 mmol/L sodium dihydrogen phosphate solution （pH 2.7） as the running buffer, at the operating voltage of 10 kV and the detection wavelength of 218 nm. It was linear for vitamin BT in the range of 1--40 μg/ml. The average recovery was 99.6 %, with RSD of 1.9 %.