基于高通量测序解析碳化温度对麻秆电极微生物群落影响

Influence of Carbonization Temperature on Bacterial Community of the Biological Carbon Electrode Based on High-throughput Sequencing Technology

采用Solixa高通量测序技术比较了1 000℃和1 800℃碳化麻秆制备电极OCP-1000和OCP-1800在生物电化学系统运行过程中形成的生物膜微生物群落结构差异.测序分别获得OCP-1000和OCP-1800附着生物膜16S rRNA基因V3区优化序列4 231和5 263条,经过97%相似度归并后获得OTUs数量分别为1 187和1 338个.α多样性指数(Chao、Shannon指数)分析表明碳化温度越低,电极生物膜微生物多样性越丰富.电极OCP-1000和OCP-1800生物膜中优势菌群均为Proteobacteria、Firmicutes和Bacteroidetes,这3个门的细菌在OCP-1000生物膜含量分别为66%、10%和9%,但在电极OCP-1800生物膜中含量分别为71%、7%和9%.不同碳化温度制备的麻秆电极在生物电化学系统运行过程形成的生物膜不仅存在共有细菌种类,也含有独特菌种,说明电极制备过程碳化温度影响电极生物膜微生物群落结构.

Microbial diversity of anodic biofilm in bioelectrochemical systems with hemp rod carbonized at 1 000 and 1 800℃ as anode was investigated using Solexa high-throughput sequencing technology. The results showed that a total of 4 231 and 5 263 optimized 16S rRNA gene sequences were gained from the electrode biofilm on the hemp rod carbonized at 1 000 and 1 800℃ , respectively. At the level of 97% similarity, 1 187 and 1 338 OTUs were obtained for electrode biofilm carbonized at 1 000 and 1 800℃ , respectively. The result of a diversity analysis showed that microbial diversity increased with decreasing carbonization temperature. Dominant phylum for both biofilms were Proteobacteria, Firmicutes and Bacteroidetes, which accounted for 66% , 10% and 9% , respectively for 1 000℃, while 71% , 7% and 9% , respectively for 1 800℃. Beside the coexisting phylum, some unique species were also discovered, demonstrating that carbonization temperature did not only influence the electrode structure, but also affected the microbial community structure.