响应面法优化蚕蛹蛋白源α-葡萄糖苷酶抑制肽酶解条件

The Enzymatic Hydrolysis Condition Optimization of α-Glucosidase Inhibitory Peptides of Silkworm Pupa Protein by Response Surface Methodology

以蚕蛹蛋白为原料,使用中性蛋白酶、酸性蛋白酶、胰蛋白酶、胃蛋白酶对其进行酶解,以α-葡萄糖苷酶活性抑制率为评价指标,筛选具有最佳α-葡萄糖苷酶抑制活性的酶品种。通过酶解温度、时间、p H值、酶底比和水底比来选出最佳单因素酶解条件,再通过部分因子试验和中心试验设计的响应面优化法进行酶解条件优化。结果最佳酶解工艺条件:酸性蛋白酶,酶解温度36.4℃,p H 3.79,酶解时间4.6 h,酶底比(质量分数)2%,水底比15 m L/g。验证试验的酶解产物质量浓度在5.0 mg/m L时,α-葡萄糖苷酶抑制率为(65.4±1.3)%。预测值与实际验证值准确性达到97.9%,所得模型具有极好的准确性。

The hydrolysis conditions of α-Glucosidase inhibitory peptides of silkworm pupa protein by response surface methodology was performed. The optimized conditions were as follows： enzyme dosage 2.0%, hydrolyzing 4.6hours at the enzymolysis temperature 36.4 ℃ and p H 3.79 with a ratio of water/substrate 15 ∶1, the α-Glucosidase inhibitory rate of silkworm pupa protein peptide reached（65.4±1.3）%（n=3） in 5.0 mg/m L. A response surface optimized model based on the α-Glucosidase silkworm pupa protein peptide extraction conditions was established. The verified test results showed the model with a 97.9% accurate rate.