瑞舒伐他汀对心肌梗死大鼠左心房交感神经重构的影响被引量：2

Effects of Rosuvastatin on Sympathetic Neural Remodeling of Left Atrium in Rats after Myocardial Infarction

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摘要目的:探讨瑞舒伐他汀对心肌梗死(myocardial infarction,MI)大鼠左心房交感神经重构的影响及其作用机制。方法:将60只雄性Wistar大鼠随机均分为3组:假手术组(Sham组),MI+瑞舒伐他汀干预组(Rstatin组)及MI组。采用免疫组化法分析3组大鼠左侧星状神经节(left stellate ganglion,LSG)及左心房组织中神经生长因子(nerve growth factor,NGF)和生长相关蛋白43(growthassociated protein 43,GAP-43)阳性物质含量以反映神经纤维的分布和密度,采用Western blot法检测两者的蛋白含量。结果:Rstatin组和MI组大鼠LSG与左心房组织中GAP-43及NGF阳性神经密度均明显高于Sham组,差异有统计学意义(P<0.01);但Rstatin组明显低于MI组,差异有统计学意义(P<0.01)。Rstatin组和MI组大鼠LSG及左心房组织中GAP-43蛋白表达均明显高于Sham组,差异有统计学意义(P<0.01);但Rstatin组明显低于MI组,差异有统计学意义(P<0.01)。Rstatin组和MI组大鼠LSG及左心房组织中NGF蛋白表达均明显高于Sham组,差异有统计学意义(P<0.01);但Rstatin组与MI组的差异无统计学意义(P>0.05)。结论:瑞舒伐他汀可有效改善MI大鼠左心房交感神经重构,其机制可能与直接抑制LSG内GAP-43及左心房组织中NGF的表达及蛋白生成有关。 OBJECTIVE： To investigate the effects of rosuvastatin on sympathetic neural remodeling of left atrium（ LA）in rats after myocardial infarction（ MI） and the mechanism of action. METHODS： 60 male wistar rats were randomly divided into sham operation group（ sham group）,MI ＋ rosuvastatin intervention group（ rstatin group）,and MI group（ MI group）.Immunohistochemistry was used to analyze the left stellate ganglion（ LSG） in rats and nerve growth factor（ NGF） in atrial tissue,positive materials of growthassociated protein 43（ GAP-43）,and then the distribution and density of nerve fiber were reflected. The expression levels of the proteins were quantified by Western blotting. RESULTS： LSG in rats,GAP-43 in atrial tissue and positive nerve density of NGF in rstatin group and MI group were significantly higher than those of sham group,with statistically significant difference（P〈0. 01）; yet rstatin group were lower than MI group,with statistically significant difference（P〈0. 01）. The protein expression of LSG and GAP-43 in rstatin group and MI group were significantly higher than those of sham group,with statistically significant difference（P〈0. 01）; yet rstatin group were lower than MI group,with statistically significant difference（P〈0. 01）. Meanwhile,the protein expression of LSG and NGF were significantly higher than those of sham group,with statistically significant difference（P〈0. 01）; yet there was no significance between rstatin group and MI group（P〈0. 05）. CONCLUSIONS： Rosuvastatin could effectively improve the sympathetic neural remodeling of LA in MI rats. The mechanism might be associated with the direct inhibition of expression and protein synthesis of GAP-43 in LSG and NGF in LA.

作者路清华顾明明魏长春路鹏刘锦

机构地区山东省泰山医院药剂科山东省宁阳县第一人民医院中医科山东省泰山医院护理部山东省泰山医院心内科

出处《中国医院用药评价与分析》 2016年第5期624-628,共5页 Evaluation and Analysis of Drug-use in Hospitals of China

基金山东医药卫生科技发展计划项目(No.2013WS0194)

关键词心肌梗死瑞舒伐他汀交感神经重构 Myocardial infarction Rosuvastatin Neural remodeling

分类号 R972.6 [医药卫生—药品]

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参考文献17

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