注射用苦参碱对阿霉素致大鼠心脏重构的影响

Effects of matrine on cardiac remodeling in rats induced by Adriamycin

目的研究注射用苦参碱对阿霉素引起大鼠心脏重构的影响。方法将52只大鼠随机分为:对照组、模型组、苦参碱(100 mg/kg)组,尾iv阿霉素制备心肌损伤模型,采用超声心动图检测大鼠心脏左心室舒张末期内径(LVEDd)、左室射血分数(LVEF)和缩短分数(FS);生理记录仪检测大鼠左心室舒张末期压(LVEPD)、收缩压(LVPS)、室内压最大收缩/舒张速率(±dp/dtmax);称大鼠体质量,脊髓离断法处死大鼠,称大鼠心脏质量并计算心脏指数;试剂盒法检测大鼠心脏组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)水平;应用流式细胞术检测大鼠心肌细胞凋亡水平。结果与模型组比较,苦参碱组大鼠LVEDd值显著降低、EF和FS值显著升高;LVEPD显著降低,LVPS、dp/dtmax及-dp/dtmax均显著升高;心脏指数、心肌细胞凋亡率显著降低;心肌细胞内GSH-Px、SOD活性明显升高,MDA水平显著降低。结论注射用苦参碱减轻阿霉素引起的心肌细胞凋亡,改善心脏重构及心脏功能,可能与改善心肌细胞氧化应激水平有关。

Objective To study the regulation ofmatrine on adriamycin-induced cardiac remodeling in rats. Methods Sixty SD rats were randomly divided into control group, model group, and matrine group. The cardiac LVEDd, LVEF, and FS values of three groups were detected by echocardiography. The values of LVEPD, LVPS, ＋dp/dtmax were detected by physiological recorder. The weights of rats were measured. The rats were killed by the method of spinal cord transection, the weight of the heart of rat was measured and the heart index was calculated. The levels of oxidative stress were evaluated by using reagent kit of glutathione peroxidase （GSH-Px）, total superoxide dismutase （SOD）, and malondialdehyde （MDA）. Flow cytometry is used to test the apoptosis of rat myocardial cells. Results Compared with model group, the value of LVEDd in matrine group decreased significantly, the value of EF and FS increased significantly; The value of LVEPD decreased significantly, the values of LVPS, dp/dtmax, and -dp/dtmax increased significantly; The heart index and myocardial apotosis levels significantly decreased; The expression levels of SOD and GSH were decreased, and MDA content was significantly increased. Conclusion Matrine reduced the apoptosis of cardiac cells induced by adriamycin in rats and improved cardiac remodeling and function which changes may be caused by oxidative stress levels improvement.