人工培养的兰坪虫草子实体中核苷类成分研究

Nucleosides from the Fruiting Body of Cultured Ophiocordyceps lanpingensis

兰坪虫草(Ophiocordyceps lanpingensis)产于我国滇西北地区,是一种寄生在钩蝠蛾幼虫上的虫生真菌。本文以四种不同培养基培养的兰坪虫草子实体为原料,采用高效液相色谱法(HPLC)同时检测10种核苷类成分(尿嘧啶、尿苷、2'-脱氧尿苷、肌苷、鸟苷、腺嘌呤、胸腺、腺苷、2'-脱氧腺苷、虫草素),并将其与野生冬虫夏草(Ophiocordyceps sinensis)、野生兰坪虫草进行对比分析,初步评估人工培养兰坪虫草子实体的质量。结果表明,以大米和蚕蛹粉为主要原料的培养基所培养的兰坪虫草子实体生长时间平均为40 d,周期短于其他三种培养方式;且其腺苷、总核苷含量分别为2744μg/g和8015μg/g,明显高于野生冬虫夏草、野生兰坪虫草,有较好的质量水平。因此,人工培养的兰坪虫草子实体具有重要的研究价值和应用潜力。

Ophiocordyceps lanpingensis, an entomogenous fungus found in Northwestern Yunnan, China, is a parasite of Hepialidae larvae. A rapid high-performance liquid chromatography（HPLC） method was developed to simultaneously determine the content of ten nucleosides（uracil, inosine, 2＇-deoxyuridine, uridine, guanosine, adenosine, adenine, thymidine, 2＇-deoxyadenosine, and cordycepin） in the Ophiocordyceps lanpingensis fruiting body, cultured in four different media. To conduct a preliminary quality evaluation of the cultured Ophiocordyceps lanpingensis fruiting body, it was compared with wild Ophiocordyceps sinensis. Ophiocordyceps lanpingensis cultured in a medium with rice and silkworm chrysalis powder as the major ingredients had an average growth period of 40 days, which was shorter than that observed with three other culture media. Additionally, adenosine and total nucleoside contents in the cultured Ophiocordyceps lanpingensis fruiting body were 2744 and 8015 μg/g, respectively, which were considerably higher than those in wild Ophiocordyceps sinensis and Ophiocordyceps lanpingensis. Thus, the fruiting body of cultured Ophiocordyceps lanpingensis has significant research value and application potential.