摘要
本实验显示,丹参的6种水溶性成分在10μmol/L能不同程度地抑制黄嘌呤─氧化酶系统产生超氧阴离子(O ̄-_2)。丹参7种水溶成分(100μmol/L)显著阻止过氧化氢(H_2O_2)所致的小鼠红细胞溶血和丙二醛(MDA)形成,其中SalA,SalB,SalC和RosA的活性较强,在1~100μmol显示良好的量效关系。在离体大鼠心脏,SalA20μmol/L能显著降低缺氧45min后再给氧30min时心肌MDA含量。结果表明,丹参中除丹参素和原儿茶醛外,还有其它活性更强的抗氧化成分。SalA对心肌缺血/再给氧损伤的保护作用可能与其氧自由基清除活性有关。
The effects of water─
soluble components isolated from Salvia miltiorrhiza on oxygen free
radical prodction and lipid peroxidation were estimated. Five
components(10 μmol/L)were shown to inhibit superoxide anion
generation by xanthine─xanthine oxidase system. It was also
demonstrated that six components( 100 μmol/L) prevented H_2O_2─
induced hemolysis and MDA fonnation in mouse erythrocytes.The
effects of Sal A, Sal B, Sal C and Ros A were dose dependent. In
Langendorff rat heart, pretreatment with Sal A 20μmol/L
significantly prevented MDA production induced by 30 min
reoxygenation after 45 min anoxia.The results indicated that there
were also other potent antioxidant componentts in Salvia milliorrhiza
besides Dphl and Pal. The protection of Sal A against myocardial
anoxiareoxygenation inury may be mainly ascribed to its oxygen free
radical scavenging activity.
关键词
丹参
丹酚酸A
心肌缺血
再灌注
原儿茶醛
自由基
过氧化脂质
Salvia miltiorrhiza
Salvianolic acid A
Myocardial anoxiareoxygenation
Free radicals
Lipid peroxide