丹参水溶性成分抗氧自由基和脂质过氧化作用

Effects of Water─soluble Components Isolated from Salviamilltiorrhiza on Oxygen Free Radical Generation and LipidPeroxidation

本实验显示，丹参的６种水溶性成分在１０μｍｏｌ／Ｌ能不同程度地抑制黄嘌呤─氧化酶系统产生超氧阴离子（Ｏ￣－＿２）。丹参７种水溶成分（１００μｍｏｌ／Ｌ）显著阻止过氧化氢（Ｈ＿２Ｏ＿２）所致的小鼠红细胞溶血和丙二醛（ＭＤＡ）形成，其中ＳａｌＡ，ＳａｌＢ，ＳａｌＣ和ＲｏｓＡ的活性较强，在１～１００μｍｏｌ显示良好的量效关系。在离体大鼠心脏，ＳａｌＡ２０μｍｏｌ／Ｌ能显著降低缺氧４５ｍｉｎ后再给氧３０ｍｉｎ时心肌ＭＤＡ含量。结果表明，丹参中除丹参素和原儿茶醛外，还有其它活性更强的抗氧化成分。ＳａｌＡ对心肌缺血／再给氧损伤的保护作用可能与其氧自由基清除活性有关。

The effects of water─ soluble components isolated from Salvia miltiorrhiza on oxygen free radical prodction and lipid peroxidation were estimated. Five components(10 μmol/L)were shown to inhibit superoxide anion generation by xanthine─xanthine oxidase system. It was also demonstrated that six components( 100 μmol/L) prevented H_2O_2─ induced hemolysis and MDA fonnation in mouse erythrocytes.The effects of Sal A, Sal B, Sal C and Ros A were dose dependent. In Langendorff rat heart, pretreatment with Sal A 20μmol/L significantly prevented MDA production induced by 30 min reoxygenation after 45 min anoxia.The results indicated that there were also other potent antioxidant componentts in Salvia milliorrhiza besides Dphl and Pal. The protection of Sal A against myocardial anoxiareoxygenation inury may be mainly ascribed to its oxygen free radical scavenging activity.