摘要
Changes in the surface structure of cell membrane and the contents of membrane pro- teins and nuclear DNA of human gastric cancer (BGC-823) cells treated with organotin compound (Et_2SnCl_2phen) were studied with a scanning electron microscope (SEM),a scanning tunneling,micro- scope (STM),and a cytofluorophotometer.It was found that Et_2SnCl_2Phen not only inhibited the cell growth but also remarkably changed the surface structure of the membrane of cancer cells.The surface of Et_2SnCl_2phen treated cancer cells was relatively smooth and showed fewer microvilli under SEM. STM images showed an uneven and loose distribution of the surface of the cell.In comparison with the untreated cancer cells,there was an evident decrease in the content of membrane proteins and nuclear DNA in Et_2SnCl_2phen treated cells.
利用扫描电镜、描隧道显微镜及细胞荧光光度计,研究了有机锡化合物Et_2SnCl_2phen 对人胃癌细胞(BGC-823)膜表面结构、膜蛋白和核中 DNA 含量的影响。发现Et_2SnCl_2phen 不但能抑制细胞生长,而且能明显改变癌细胞膜表面结构。电镜下显示出癌细胞表面比较光滑和较少微绒毛。扫描隧道显微镜的图像显示出细胞表面不平和疏松分布。与对照组相比,经 Et_2SnCl_2phen 处理的细胞膜蛋白质和核中 DNA 的含量明显减少。
